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Method for producing acetone butanol by steam explosion straw xylose fermentation and extracting remainder

A technology for steam explosion of straw and acetone butanol, which is applied in the field of straw resource utilization, can solve the problems of affecting the normal growth of acetone butanol, low economic benefits, environmental pollution and the like, and achieves the effect of improving the comprehensive utilization value.

Active Publication Date: 2011-01-12
北京中科百瑞能工程技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, acid hydrolysis is mostly used to extract xylose from straw. Although it can reach the substrate concentration of xylose fermented by acetone butanol, it is easy to produce acid pollution and a large amount of fermentation inhibitors, which will affect the normal growth of subsequent acetone butanol.
In addition, the fermentation waste residue containing a large amount of bacterial protein has not been fully utilized, resulting in environmental pollution and low economic benefits.

Method used

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  • Method for producing acetone butanol by steam explosion straw xylose fermentation and extracting remainder

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Cut the corn stalks into 3-5cm sections, add 1 times of tap water and fully soak them for 10 minutes, put them into the steam explosion tank, pressurize them under the pressure of 1.5MPa for 5 minutes, and then release them rapidly from the reactor to obtain the steam explosion straws; add 2 double tap water, soak at 60°C for 20 minutes, and separate the steam-exploded straw liquid and steam-exploded straw solid with an extruder; after the steam-exploded straw liquid is filtered, it is distilled under reduced pressure at 0.9MPa and 60°C, and the distillate is separated and concentrated Obtain furfural and acetic acid; add xylanase to the residual liquid after distillation, and hydrolyze it at 50°C for 12 hours. The hydrolyzed liquid is successively treated with macroporous adsorption resin and basic anion exchange resin, and the xylose concentration of the obtained steam-exploded straw is 5%. , then adjust the pH to 6.5 with NaOH, add 3% nitrogen source, and 0.01% trace ...

Embodiment 2

[0033]Cut the corn stalks into 3-5cm sections, add 1 times of tap water and fully soak them for 10 minutes, put them into the steam explosion tank, pressurize them under the pressure of 1.5MPa for 5 minutes, and then release them rapidly from the reactor to obtain the steam explosion straws; add 3 double tap water, soak at 60°C for 20 minutes, and separate the steam-exploded straw liquid and steam-exploded straw solid with an extruder; after the steam-exploded straw liquid is filtered, it is distilled under reduced pressure at 0.9MPa and 60°C, and the distillate is separated and concentrated Obtain furfural and acetic acid; add xylanase to the remaining liquid after distillation, and hydrolyze it at 50°C for 12 hours. The hydrolyzed liquid is successively treated with macroporous adsorption resin and basic anion exchange resin, and the xylose concentration of the obtained steam-exploded straw is 4%. , then adjust the pH to 6.5 with NaOH, add 3% nitrogen source, and 0.01% trace ...

Embodiment 3

[0035] Cut the corn stalks into 3-5cm sections, add 1 times of tap water and fully soak them for 10 minutes, put them into the steam explosion tank, pressurize them under the pressure of 1.5MPa for 5 minutes, and release them from the reactor rapidly to obtain the steam explosion straws; add 4 double tap water, soak at 60°C for 20 minutes, and separate the steam-exploded straw liquid and steam-exploded straw solid with an extruder; after the steam-exploded straw liquid is filtered, it is distilled under reduced pressure at 0.9MPa and 60°C, and the distillate is separated and concentrated Obtain furfural and acetic acid; add xylanase to the residual liquid after distillation, and hydrolyze it at 50°C for 12 hours. The hydrolyzed liquid is successively treated with macroporous adsorption resin and basic anion exchange resin, and the xylose concentration of the obtained steam-exploded straw is 3%. , then adjust the pH to 6.5 with NaOH, add 3% nitrogen source, and 0.01% trace eleme...

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Abstract

The invention relates to a method for producing acetone butanol by steam explosion straw xylose fermentation and extracting remainder, which comprises the following steps of: performing solid and liquid separation after steam explosion straws are soaked into water, and obtaining furfural, organic acid and xylose from the steam explosion straw liquid; producing the acetone butanol by the steam explosion straw xylose fermentation, wherein the yield of the total solvent is 12 to 22g / L; and extracting the steam explosion straw solid by using alkali, wherein the alkali extraction liquid is used for preparing lignin and the alkali extraction residue is used for making paper and producing feed by fermentation. The method transforms cellulose, hemicellulose and lignin in the straws by stages, canproduce furfural, organic acid, xylose, lignin and papermaking fiber, and can produce fermentation products such as acetone, butanol, feed protein and the like by biotransformation. The method realizes comprehensive utilization of straw resources, has no waste and pollutant discharge during production, achieves pollution-free production, and has good social benefit and economic benefit.

Description

technical field [0001] The method belongs to the field of straw resource utilization, and in particular relates to a method for steam-exploding straw xylose to ferment acetone butanol and extract residues. Background technique [0002] Acetone, butanol, and ethanol produced by the fermentation of Clostridium.acetobutylicum (Clostridium.acetobutylicum) are collectively referred to as the total solvent of acetobutylicum. They are important organic solvents and chemical raw materials, and are widely used in spray paint, explosives, plastics, pharmaceuticals, vegetable oil extraction, and organic glass. , synthetic rubber and other industries. In particular, butanol is a new type of biofuel with great potential. It has excellent characteristics such as high calorific value, easy miscibility, high octane number and low volatility, and its performance is better than ethanol in replacing gasoline as a fuel. [0003] Acetone-butanol fermentation was once the second largest fermenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/36C12P7/16C07D307/50C07G1/00D21C1/02D21C1/06C12S3/04A23K1/14C12R1/145A23K10/37
CPCY02P60/87
Inventor 陈洪章王岚
Owner 北京中科百瑞能工程技术有限责任公司
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