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Bactericide for generating methane and preparation technology thereof

A technology of methanogens and technology, applied in the directions of bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems such as influence, instability of biogas production, and restriction of biogas development.

Inactive Publication Date: 2010-12-22
刘秀丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After World War II, biogas fermentation technology was developed in some countries in Western Europe, but it was affected by the influx of cheap oil into the market
However, due to the different raw materials such as climate, crop straw, livestock and poultry manure, etc., the ingredients are very different, coupled with the differences in user operation technology, the biogas production is unstable, the gas production is not balanced in spring, summer and autumn, and the cold season in the north is uneven. Gas production and other phenomena restrict the development of biogas to a certain extent

Method used

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  • Bactericide for generating methane and preparation technology thereof
  • Bactericide for generating methane and preparation technology thereof
  • Bactericide for generating methane and preparation technology thereof

Examples

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Effect test

example 1

[0023] A methanogenic bacterium agent and its preparation method, the mass ratio of the methanogenic bacterium Clostridium cellulosus: Clostridium beijerinckii: Methanogenic Lithium jahnii: Methanobacillus vortii: thermophilic methane sarcasia Coccus: Methanosarcina pasteuri is 2:3:1:1:1:4.

[0024] The methanogenic bacteria agent is composed of 6 kinds of microorganisms, belonging to 6 strains of microorganisms, respectively Clostridium thermocellum Viljoen et al., Clostridium beijerinckii Donkeremend.Keis et al., Clostridium beijerinckii Donkeremend.Keis et al. Methanococcus jannaschii (Jones et al.) Whitman], Methanothermobacter wolfeii (Winter et al.) Wasserfallen et al.], Methanosarcina thermophila Zinder et al. al.) and Methanosarcina barkeri Schnellen. The preservation numbers are respectively: Clostridium thermocellum Viljoen et al. is preserved in China Agricultural Microorganism Culture Collection Management Center, and its number is ACCC00026; Clostridium beijerinc...

example 2

[0040] Clostridium thermocellum: Clostridium bejejejerinckii: Methanosarcina jannaisi: Methanosarcina worriii: Methanosarcina thermophila: Methanosarcina pasteurii: 1:1:1:1:1:1 : 1.

[0041] The methanogenic bacteria agent is composed of 6 kinds of microorganisms, belonging to 6 strains of microorganisms, respectively Clostridium thermocellum Viljoen et al., Clostridium beijerinckii Donkeremend.Keis et al., Clostridium beijerinckii Donkeremend.Keis et al. Methanococcus jannaschii (Jones et al.) Whitman], Methanothermobacter wolfeii (Winter et al.) Wasserfallen et al.], Methanosarcina thermophila Zinder et al. ) and Methanosarcina barkeri Schnellen. The preservation numbers are respectively: Clostridium thermocellum Viljoen et al. is preserved in the China Agricultural Microorganism Culture Collection Management Center, and its number is ACCC00026; Clostridium beijerinckii Donkeremend.Keis et al. is preserved in China The Agricultural Microbial Culture Collection Management C...

example 3

[0056] Clostridium thermocellum: Clostridium beijerinckii: Methanosarcina jannaisi: Methanosarcina worriii: Methanosarcina thermophila: Methanosarcina pasteurii: 2:2:1:1:1:2 .

[0057] The methanogenic bacteria agent is composed of 6 kinds of microorganisms, belonging to 6 strains of microorganisms, respectively Clostridium thermocellum Viljoen et al., Clostridium beijerinckii Donkeremend.Keis et al., Clostridium beijerinckii Donkeremend.Keis et al. Methanococcus jannaschii (Jones et al.) Whitman], Methanothermobacter wolfeii (Winter et al.) Wasserfallen et al.], Methanosarcina thermophila Zinder et al. al.) and Methanosarcina barkeri Schnellen. Its preservation numbers are respectively: Clostridium thermoceilum Viljoen et al. is preserved in China Agricultural Microorganism Culture Collection Management Center, and its number is ACCC00026; Clostridium beijerinckii Donkeremend.Keis et al. is preserved in China Agricultural Microorganism Culture Collection Management Center, ...

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Abstract

The invention relates to a microbial agent and a preparation technology thereof, belonging to the technical filed of agricultural production. In the preparation technology, Clostridium thermocellum, Clostridium beijerinckii, Methanocaldococcus jannaschii, Methanothermobacter wolfeii, Methanosarcina thermophila and Methanosarcina barkeri Schnellen are coordinated in mass ratio of 1-2:1-3:1:1:1:1-4, the total number of effective viable bacteria, the operation is carried out in an asepsis condition, and the product is kept is a dark place. To a house hold fermenting tank, a bactericide for generating methane is required to be added, the additive amount accounts for 0.1-0.2% of the feeding amount; to a semi-batch (semi-continuous) fermentation mode and a continuous fermentation mode, the additive amount accounts for 0.05-0.1% of the feeding amount. The bactericide for generating methane has wide adaptability to temperature, can generate methane continuously from 30 DEG C to 85 DEG C, and does not have strict requirements for organic matter materials. The technology can be used for household small-size methane tanks and large-scale industrial methane apparatuses to use.

Description

technical field [0001] The invention relates to an agricultural microbial bacterial agent and its preparation technology. The invention belongs to the technical field of agricultural production. This method can effectively improve the conversion and utilization rate of organic materials in biogas raw materials, accelerate the generation of biogas, increase the amount of biogas produced, and is safe and environmentally friendly. It has good ecological benefits and considerable social benefits. This technology can be applied to household small biogas digesters and large factory biogas facilities. Background technique [0002] Biogas was discovered by Italian A. Volta in the swamp in 1776. In 1916, Russia В.П. Omeryansky isolated the first strain of methanogen (but not pure). China successfully isolated Methanosarcina for the first time in 1980. At present, more than 20 strains of methane bacteria have been isolated in the world. [0003] The world's first biogas generato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12P5/02C12R1/01C12R1/145
CPCY02E50/343Y02E50/30
Inventor 刘秀丽
Owner 刘秀丽
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