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Preparation method of electrochemical immunosensor for detecting salbutamol quickly

An immunosensor, albuterol technology, applied in the field of chemical detection, can solve the problems of high detection cost, cumbersome operation process, long detection time, etc., and achieve the effects of easy fixation, high activity, and promotion of electron transfer.

Inactive Publication Date: 2010-12-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the methods for detecting salbutamol residues mainly include chromatography, chromatography-mass spectrometry, immunoassay, and microbial methods, but the above methods have high detection costs, cumbersome operation, long detection time, and are not suitable for on-site large-throughput screening. shortcoming

Method used

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  • Preparation method of electrochemical immunosensor for detecting salbutamol quickly
  • Preparation method of electrochemical immunosensor for detecting salbutamol quickly
  • Preparation method of electrochemical immunosensor for detecting salbutamol quickly

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Step 1, pretreatment of the electrode: use the glassy carbon electrode on the suede with AL 2 o 3 Polish the powder to the mirror surface, rinse with distilled water, and then use ultrasonic cleaning for 10 minutes; put the cleaned glassy carbon electrode into nitric acid and acetone solution with a volume ratio of 1:1 and soak for 15 minutes each, and then rinse the electrode with distilled water; The electrodes are dried under infrared lamps.

[0026] Step 2, preparation of nano-gold colloid solution and carbon nanotube suspension: Measure 50 mL of 0.01% chloroauric acid solution in a flask, heat to boiling in a microwave synthesizer (400W, 100°C), add 3 mL of freshly prepared 1% trisodium citrate aqueous solution, stirred vigorously and kept in a slightly boiling state, observed the color of the solution from colorless to deep purple, and then to purple red, disconnected the power after about 5 minutes, cooled naturally, and placed the prepared sol in the refrigerat...

Embodiment 2

[0034] Step 1, pretreatment of the electrode: polishing the glassy carbon electrode on the suede and rinsing with distilled water is the same as in Example 1, and then cleaning it with ultrasonic waves for 20 minutes; Soak in nitric acid and acetone solutions for 10 minutes each, then rinse the electrode with distilled water; dry the electrode under an infrared lamp.

[0035] Step 2, preparation of nano-gold colloid solution and carbon nanotube suspension: Measure 50 mL of 0.01% chloroauric acid solution in a flask, heat to boiling in a microwave synthesizer (400W, 100°C), add 5 mL of freshly prepared 1% trisodium citrate aqueous solution, stirred vigorously and kept in a slightly boiling state, observed the color of the solution from colorless to deep purple, and then to purple red, disconnected the power after about 5 minutes, cooled naturally, and placed the prepared sol in the refrigerator for 4 Store at ℃ (the obtained colloidal gold is dark red, and the average diameter ...

Embodiment 3

[0040] Step 1, pretreatment of the electrode: polishing the glassy carbon electrode on the suede and rinsing with distilled water is the same as in Example 1, and then cleaning it with ultrasonic waves for 30 minutes; Soak in nitric acid and acetone solutions for 10 minutes each, then rinse the electrode with distilled water; dry the electrode under an infrared lamp.

[0041] Step 2, preparation of nano-gold colloid solution and carbon nanotube suspension: Measure 50 mL of 0.01% chloroauric acid solution in a flask, heat to boiling in a microwave synthesizer (400W, 100°C), add 3 mL of freshly prepared 1% trisodium citrate aqueous solution, stirred vigorously and kept in a slightly boiling state, observed the color of the solution from colorless to deep purple, and then to purple red, disconnected the power after about 5 minutes, cooled naturally, and placed the prepared sol in the refrigerator for 4 Store at ℃ (the obtained colloidal gold is dark red, and the average diameter ...

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Abstract

The invention discloses a preparation method of an electrochemical immunosensor for detecting salbutamol quickly, which belongs to the technical field of chemical detection. In the preparation method, a glassy carbon electrode is modified by carbon nanotubes, polythionine and nano gold in turn, and the electrochemical immunosensor is obtained by a self-assembly process. The invention can prepare the electrochemical immunosensor which has high sensitivity and stability and can detect salbutamol on site quickly.

Description

technical field [0001] The invention relates to a method in the technical field of chemical detection, in particular to a method for preparing an electrochemical immunosensor for rapid detection of albuterol. Background technique [0002] Salbutamol is a β-receptor stimulant, which can make the nutrients in the animal body transfer from fat to muscle, showing a nutrient redistribution effect, and then regulate the material metabolism of the animal body, enhance fat decomposition, promote protein synthesis, and significantly improve carcass leanness. rate and feed remuneration. However, after humans eat animal food with albuterol residues, poisoning will occur in varying degrees. Their abuse and residues in animal foods seriously endanger people's health and life safety, and seriously affect my country's livestock and poultry products export trade. Therefore, my country, the European Union and most countries in the world have expressly banned albuterol from being used as a f...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/38G01N27/48G01N33/53
Inventor 王正武米芹
Owner SHANGHAI JIAO TONG UNIV
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