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Method for extracting soybean protein by using dodecyltrimethylammonium chloride reverse micellar solution

A technology of dodecyl trimethyl ammonium chloride and soybean protein is applied in reverse micelle technology and its application field, which can solve the problems of long time consumption, low extraction rate, consumption and the like, so as to shorten the extraction time and maintain biological The effect of active, high product purity

Active Publication Date: 2010-12-15
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the method of using reverse micellar extraction technology to simultaneously separate soybean protein and oil (CN 101343309 A), the extraction process uses mechanical stirring, and its disadvantages are: (1) It takes a long time, and the entire extraction process takes about 4 hours; (2) ) The extraction rate is low, around 50%-60%
Although the AOT / isooctane reverse micellar system has certain advantages, due to the shortcomings of AOT’s low safety and difficult separation with the product, the optimization of extraction technology and the application of other reverse micellar systems to plant proteins research is necessary

Method used

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  • Method for extracting soybean protein by using dodecyltrimethylammonium chloride reverse micellar solution
  • Method for extracting soybean protein by using dodecyltrimethylammonium chloride reverse micellar solution

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Add 100 g of pretreated full-fat soybean flour into 4 L of reverse micelles solution with DTAC concentration of 70 g / L, control conditions, the buffer in the reverse micelles solution is KCl-phosphate buffer, control Condition W 0 40. KCl concentration is 0.05 mol / L, pH is adjusted to 7.0, extraction temperature is 45°C, sonicated at 180w for 20min, then centrifuged at 4500r / min for 12min to remove residue, the obtained supernatant is the pre-extract ， The extraction rate of protein was 95.83%. Add an equal volume of 1.0 mol / L KCl-phosphate buffer solution to adjust the pH value to 6.0, sonicate in an ultrasonic cleaner for 30 minutes at a power of 180 W, and then centrifuge for 10 minutes, and the lower layer solution is the protein aqueous solution. The post-extraction rate of protein is 98.64%. Overall, the protein extraction rate is 94.53%.

Embodiment 2

[0034] 35g of pretreated full-fat soybean powder was added to 1L, DTAC concentration in the reverse micelles solution of 80g / L, the buffer in the reverse micelles solution was KCl-phosphate buffer, the control condition W 0 38. The concentration of KCl is 0.05 mol / L, the pH is adjusted to 7.0, the extraction temperature is 40°C, and the power of 210w is sonicated for 15min. Then centrifuge at a speed of 5000r / min for 10 minutes to remove the residue, and the resulting supernatant is the pre-extraction ， The extraction rate of protein was 93.72%.

[0035]Add an equal volume of 1.0 mol / L KCl-phosphate buffer solution to adjust the pH value to 6.0, sonicate in an ultrasonic cleaner for 25 minutes at a power of 200 W, and then centrifuge for 10 minutes. The lower solution is the protein aqueous solution. The post-extraction rate of protein is 98.03%. Overall, the protein extraction rate is 91.87%.

Embodiment 3

[0037] Put 100 g of pretreated soybean meal into 4 L of 70 g / L DTAC reverse micelles solution, control conditions, the buffer in the reverse micelles solution is KCl-phosphate buffer, control conditions W 0 40, the concentration of KCl is 0.05 mol / L, the pH is adjusted to 7.0, the extraction temperature is 45°C, and the power of 180w is sonicated for 20min. Then centrifuge at a speed of 4500r / min for 12 minutes to remove the residue, and the resulting supernatant is the pre-extraction ， The extraction rate of protein was 97.24%.

[0038] Add an equal volume of 1.0 mol / L KCl-phosphate buffer solution to adjust the pH value to 6.0, sonicate in an ultrasonic cleaner for 30 minutes at a power of 180 W, and then centrifuge for 10 minutes, and the lower layer solution is the protein aqueous solution. The post-extraction rate of protein is 98.80%. Overall, the protein extraction rate is 96.07%. Under the same conditions, the extraction rate of soybean protein in full-fat soybean fl...

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Abstract

The invention relates to a method for extracting soybean protein by using a dodecyltrimethylammonium chloride reverse micellar solution, which is characterized by comprising the following steps: adding full-fat soybean powder or soybean meal powder to a dodecyltrimethylammonium chloride (DTAC) reverse micellar solution, extracting, and carrying out solid-liquid separation to obtain a soybean protein solution. The invention has the following major advantages: because of the use of the dodecyltrimethylammonium chloride reverse micelle, the formation time of the reverse micellar system is short, and the preparation is more convenient and faster; because the extraction of soybean protein by DTAC / n-heptane-n-hexyl alcohol reverse micellar system is assisted by ultrasound, the preparation is more convenient and faster; the extraction rate is obviously improved, and the extraction time is shortened; in the extraction process, the protein is enclosed by water environment in the reverse micelle, the water environment is similar to the intracellular environment and the conditions are wild, thereby ensuring that the protein can not be denatured easily and the bioactivity thereof can be kept; compared with other surfactants, the residual surfactant in the soybean protein, i.e. the dodecyltrimethylammonium chloride, is easy to separate, thereby achieving high product purity; and the method has the advantages of no acid and alkaline wastewater discharge and no pollution.

Description

technical field [0001] The invention belongs to the field of reverse micelle technology and its application, and in particular relates to a method for extracting soybean protein by using dodecyltrimethylammonium chloride reverse micelle solution. Background technique [0002] Soybean protein is the most familiar, safe and economical plant protein resource in terms of nutritional composition, rich resources and processing technology. It is one of the few nutritional products that can replace animal protein. Among them, soybean protein isolate is a refined soybean protein product with high protein content. In addition to high nutritional value, it also has many important functional properties. These functional properties are of great value for the application of soybean protein in food. It can be used as an intermediate raw material for processing functional food, and is widely used in industries such as meat products, dairy products, cold food and cold drinks, baked food and...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 陈复生程小丽杨宏顺刘慧姜崇斌侯红江姚永志布冠好时冬梅宫保文张鹏龙武秋颖刘海远
Owner HENAN UNIVERSITY OF TECHNOLOGY
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