Method for screening novel restoring gene for HL cytoplasm male sterile rice

Abstract

The invention discloses a method for screening a novel restoring gene for HL cytoplasm male sterile rice. The method comprises the following steps of: firstly, designing a primer of a restoring gene coseparation molecular marker and establishing a standard polymerase chain reaction (PCR) amplification system; secondly, comparing and analyzing a molecular marker amplification banding pattern and screening a rice variety or an ecotype having different molecular markers; and finally, verifying a new restoring gene locus by using genetic analysis and determining the novel restoring gene. In the method, the conventional genetic analysis is combined with molecular genetic marker technology, and a new gene resource is developed and utilized purposefully. The method has the characteristics of simple and convenient operation, rapidness, correctness and accurate selection of a novel restorer and is favorable for expanding restoring spectrum, improving the polymerization selection efficiency of excellent recovering genes and promoting the maturing rate, yield and sustainable development of hybrid rice.

Description

technical field [0001] The present invention relates to the technical field of molecular marker detection, and more specifically to a method for rapid and qualitative detection of red lotus-type cytoplasm, which is applicable to rice red lotus-type gametoplasmic male sterility in various rice cultivars, farm species and wild rice Screening for fertility restorer genes. Background technique [0002] Plants have accumulated a large amount of variation in the genome through natural mutation or recombination during the long evolutionary process, resulting in differences among different strains or strains within the same species. This difference is manifested at the genetic level as the difference of certain DNA segments from each other. When this variation is co-segregated with a specific gene, it can be used as a unique molecular signature of the gene. If these DNA fragments are subjected to in vitro amplification, electrophoresis, and color development, bands of different si...

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Problems solved by technology

Moreover, the same restorer gene cannot restore BT-type and WA-type male sterility at the same time. Only after recombination and combining Rf1 and Rf3 in the same restorer line can they restore the corresponding cytoplasmic sterile lines. There is no additive effect of the gene, each restorer gene is inherited independently, and there is no obvious interaction effect between them

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