Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Synthesis method of 4-amino azobenzene artificial antigen

An aminoazobenzene, artificial antigen technology, applied in the direction of animal/human protein, albumin peptide, serum albumin, etc., can solve the problem of inability to detect, and achieve the effect of simple synthesis steps

Inactive Publication Date: 2010-12-01
WUXI ENTRY EXIT INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current relevant standards and regulations in foreign countries include 4-aminoaniline in the ranks of banned aromatic amines, such as the German "Food and Consumer Goods Law", etc., but domestic standards involving textiles, dyes, and leather products have not included it. Included, due to the limitations of detection technology, conventional inspection methods cannot detect it
In recent years, research on azo immunoassay methods has been carried out at home and abroad, but there is no report on the enzyme-linked immunoassay for 4-aminoazobenzene at home and abroad. In order to make up for this gap, it is necessary to prepare 4-aminoazobenzene Azobenzene artificial antigen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthesis method of 4-amino azobenzene artificial antigen
  • Synthesis method of 4-amino azobenzene artificial antigen
  • Synthesis method of 4-amino azobenzene artificial antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] (1) Preparation of artificial antigen

[0022] Take 0.1mmol of 4-aminoazobenzene and dissolve it in 2mL of 1,4-dioxane, put it in an ice bath, add 1M hydrochloric acid to adjust the pH to 2.0, put it in an ice bath, add 0.02M sodium nitrite until the starch test paper turns blue, and set it at 4°C , stirring and incubating for 30min, it is A liquid.

[0023] Bovine serum albumin BSA 0.001mmol is dissolved in 2mL, 0.05mol / L CBS buffer solution, which is solution B.

[0024] Add liquid A drop by drop to liquid B, adjust the pH to 9.0 while adding, incubate at 4°C for half an hour, and react at room temperature for 4 hours to obtain the 4-aminoazobenzene artificial antigen mixture.

[0025] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0026] Transfer the 4-aminoazobenzene artificial antigen mixture into a dialysis bag, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a synthesis method of 4-amino azobenzene artificial antigen, belonging to the technical field of biochemical engineering. The method comprises the following steps of: taking 4-amino azobenzene as hapten; coupling the 4-amino azobenzene with carrier protein BSA by means of diazotization; and measuring the coupling ratio of a coupling material by means of spectroscopic luminosity. The synthesis method successfully synthesizes the artificial antigen of the 4-amino azobenzene, is simple and effective in synthesis steps, can be completely used in immunoassay, provides the necessary artificial antigen to the later research of users, and can meet the requirement of the research of the 4-amino azobenzene in China.

Description

technical field [0001] The invention discloses a method for synthesizing a universal artificial antigen of 4-aminoazobenzene, which belongs to the technical field of biochemical engineering. Background technique [0002] Azo dyes (a class of organic compounds in which the two ends of the azo group are connected to aryl groups) are the most widely used synthetic dyes in the printing and dyeing process of textiles and clothing. Under special conditions, it can decompose and produce more than 20 carcinogenic aromatic amines. Change the DNA structure of the human body through activation to cause pathological changes and induce cancer. Azo is one of the mandatory inspection items required by international environmental protection. There are three inspection methods: Thin Layer Chromatography (TLC), Gas Chromatography and Mass Spectrometry (GC-MSD) and High Performance Liquid Chromatography (HPLC). The standard stipulates that the inspected product shall not contain 23 kinds of a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/765G01N21/31
Inventor 殷祥刚胥传来董激文林菲季晓丹宋姗姗许宙陈连君
Owner WUXI ENTRY EXIT INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com