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General trivalent gene recombination vaccine for preventing infection of leptospira interrogans of different sero-groups and preparation method thereof

A leptospira and gene recombination technology, applied in gene therapy, recombinant DNA technology, chemical instruments and methods, etc., to achieve the effects of safe and reliable preparation methods, no cross-protection, and large side effects

Inactive Publication Date: 2010-11-03
严杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to fill up the defects of existing Leptospira interrogans multivalent whole bacterial killed vaccine and multivalent outer membrane vaccine, to provide a general-purpose vaccine that can effectively prevent the infection of different serogroups Leptospira interrogans and has low manufacturing cost. Trivalent gene recombinant vaccine and preparation method thereof

Method used

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  • General trivalent gene recombination vaccine for preventing infection of leptospira interrogans of different sero-groups and preparation method thereof
  • General trivalent gene recombination vaccine for preventing infection of leptospira interrogans of different sero-groups and preparation method thereof

Examples

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Embodiment 1

[0018]Example 1. The general-purpose trivalent gene recombinant vaccine for preventing leptospirosis in this example uses genetic engineering technology. First, lipL32 / 1, lipL21 and ompL1 / 2 gene clones are respectively obtained, and then the same two flexible peptide sequences are used Link the interrogation mark Leptospira jaundice haemorrhagic group Lai type Lai strain lipL32 / 1 and lipL21 gene, lipL21 and ompL1 / 2 gene to construct three interrogation marks containing outer membrane lipoprotein LipL32 / 1, LipL21 and transmembrane protein OmpL1 / 2 Artificial Fusion Gene lipL32 / 1-lipL21-ompL1 / 2 of Leptospira Specific Surface Antigen and Its Prokaryotic Expression System E.coliBL21DE3 pET42a-lipL32 / 1-lipL21-ompL1 / 2 The purified recombinant expression product rLipL32 / 1-LipL21-OmpL1 / 2 of the expression system is used as vaccine antigen.

[0019] The general-purpose trivalent gene recombinant vaccine for preventing leptospirosis of the present invention is based on the selection of a...

Embodiment 2

[0030] Example 2. In this example, the purified recombinant expression product rLipL32 / 1-LipL21-OmpL1 / 2 is used as an antigen and aluminum hydroxide is used as an adjuvant (W:W) and mixed at a ratio of 1:10 to become a general-purpose drug for preventing leptospirosis. Type trivalent gene recombinant vaccine, through golden hamster protection test and immunized golden hamster serum-specific antibody detection, it is determined that the universal trivalent gene recombinant vaccine for preventing leptospirosis after immunizing golden hamsters by subcutaneous injection, immune golden hamsters against different Immunoprotective effects and serum antibody titers of serogroup Leptospira interrogans fatal infection.

[0031]The reason for using aluminum hydroxide as an adjuvant in the universal trivalent gene recombinant vaccine for preventing leptospirosis of the present invention is that aluminum hydroxide is a safe and effective injection adjuvant that has been used for many years....

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Abstract

The invention relates to a trivalent gene recombination vaccine for preventing leptospirosis and a preparation method thereof and aims to effectively prevent infection of leptospira interrogans of different sero-groups with low manufacturing cost. The technical scheme is that: the trivalent gene recombination vaccine for preventing the infection of leptospira interrogans of different sero-groups consists of a lipL32 / 1 gene, a lipL21 gene and an ompL1 / 2 gene cloned from a leptospira icterohemorrhagiae genome; a fusion gene lipL32 / 1-lipL21-ompL1 / 2 prepared by a technique for gene engineering has a nucleotide sequence and an amino acid sequence shown in a sequence 5; and the lipL32 / 1 gene is connected with the lipL21 gene, and the lipL21 gene is connected with the ompL1 / 2 gene through two same flexible peptides shown in a sequence 4.

Description

technical field [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a trivalent gene recombinant vaccine for preventing leptospirosis and a preparation method thereof. Background technique [0002] Leptospira interrogans, caused by the infection of the pathogenic Leptospira interrogans, is a natural foci zoonotic infectious disease prevalent in the world, especially in rice-growing areas, so our country is a Leptospira One of the main epidemic areas of the epidemic. [0003] There are many natural host animals for Leptospira interrogans. After infection, there are no symptoms or mild symptoms, and they usually do not die. However, Leptospira interrogans can be excreted from the urine, directly polluting water sources or polluting water sources through soil (infested water). Because Leptospira interrogans can survive for a long time in various water systems and can spread rapidly through infested water, leptospirosis is one of the inf...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/02A61K39/116C12N15/62C12N15/70C07K19/00A61P31/04
CPCY02A50/30
Inventor 严杰
Owner 严杰
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