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Application of ligusticum chuanxiong hort or extract thereof in preparing anti-aging products

A chuanxiong extract and anti-aging technology, which is applied in the application field of chuanxiong or its extract in the preparation of anti-aging products, can solve the problems that the research on anti-aging effect has not been reported, and achieves the effect of prolonging lifespan

Active Publication Date: 2010-11-03
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Chuanxiong is widely used in the treatment of senile diseases and has achieved good curative effect, there is no report on its anti-aging effect

Method used

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  • Application of ligusticum chuanxiong hort or extract thereof in preparing anti-aging products
  • Application of ligusticum chuanxiong hort or extract thereof in preparing anti-aging products
  • Application of ligusticum chuanxiong hort or extract thereof in preparing anti-aging products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, Chuanxiong extract on the impact test of Caenorhabditis elegans lifespan

[0024] 1 material

[0025] 1.1 strains, nematodes

[0026] Strain E.coli OP50, wild-type Caenorhabditis elegans Bristol (N 2 ) were purchased from the Nematode Genetics Center (CGC).

[0027] 1.2 Drugs and reagents

[0028] Chuanxiong extract is prepared according to the following method: select medicinal material Chuanxiong (dried rhizome of Umbelliferae Ligusticumchuanxiong Hort.), add 6 times pure water, heat, boil and keep boiling for 40 minutes, then filter Collect filtrate 1; then add 6 times of pure water to the filter residue, heat, keep boiling for 40 minutes after boiling, then filter and collect filtrate 2; mix filtrate 1 and filtrate 2, and dry the obtained powder, which is Chuanxiong Water Extract. According to the method, 1 gram of Chuanxiong water extract is prepared from 20 grams of crude drug.

[0029] Agar powder (Japan Kyowa Co., Ltd.), 5-fluorouracil (5-Flu...

Embodiment 2

[0049] Example 2, Molecular Mechanism of Ligusticum Chuanxiong Extract Prolonging the Lifespan of Caenorhabditis elegans

[0050] 1 material, instrument are with embodiment 1.

[0051] 2 Grouping of real-time fluorescence quantitative PCR (qRT-PCR) experiment: control group and medication group (the group with the strongest life-prolonging effect in life-span experiment, CXE solution with a concentration of 25 mg / L).

[0052] 3 Research on the molecular mechanism of life extension

[0053] 3.1 Extraction of nematode total RNA and cDNA synthesis

[0054] About 1,000 nematodes in the L4 stage were picked from the control group and the treatment group into the culture medium. After feeding for 10 days, the nematodes in each group were collected separately, and the total RNA was extracted by TRIZOL method, digested with RNase free DNase, and purified by TRIZOL method. Gel electrophoresis identified that the RNA was not significantly degraded, and was frozen at -80°C. Take 2 μg ...

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Abstract

The invention discloses a new application of ligusticum chuanxiong hort or an extract thereof. The ligusticum chuanxiong hort or the extract thereof has the following four new purposes: (1) application in preparing anti-aging products; (2) application in preparing gene expression products for regulating caloric restriction pathways; (3) application in preparing gene expression products for regulating insulin signaling pathways; and (4) application in preparing caenorhabditis elegans life extenders. In the invention, the caenorhabditis elegans is used as a model organism, the influence of CXE (Ligusticum Chuangxiong Extract) on caenorhabditis elegan life is observed by taking a whole animal experiment as a detection means, the CXEs of a plurality of concentrations are all found to have a role or trend of life prolongation to the caenorhabditis elegan, wherein the concentration of 25mg / L has an optimal life prolongation effect, and the average life and the longest life are both prolonged by about 15 percent. Molecular mechanism research for life prolongation by utilizing the known relevant caenorhabditis elegan aging gene and adopting a qRT-PCR (Quantitative Real Time-Polymerase Chain Reaction) method shows that the CXE prolongs the life through intervening an Insulin / IGF-1 pathway and a feeding control (caloric restriction) pathway, thereby providing new content and direction for the molecular mechanism research of the drug and also providing a new ideal and means for the research of delaying aging by using the traditional Chinese medicine.

Description

technical field [0001] The invention relates to the new application of Rhizoma Chuanxiong or its extract for antiaging, in particular to the application of Rhizoma Chuanxiong or its extract in preparing antiaging products. Background technique [0002] With the increasingly severe situation of population aging, research on aging mechanism and anti-aging drugs has become a hot spot and key issue in life sciences. The mechanism of aging is complex and there are many theories. So far, there are more than 200 theories or theories explaining the mechanism of aging, including: genetic control theory, cell telomere theory, free radical damage theory, somatic cell mutation theory, immune disorder theory, Mitochondrial damage theory, etc. . Among them, there are two main ways to delay aging, one is the insulin signaling pathway (AntebiA. Inside insulin signaling, communication is key to long life [J]. SciAgingKnowledge environ, 2004, (23): pe25); Caloric restriction pathway (Koen ...

Claims

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Application Information

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IPC IPC(8): A61K36/236A61P39/06A23L1/30A23L33/10A23L33/105
Inventor 汪晓燕王丹巧王香明于友华喻晓春李连达
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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