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Method for inducing megakaryoblast and megakaryocyte in vitro

A technique for megakaryocyte progenitor cells and megakaryocyte cells is applied in the field of inducing and differentiating mononuclear cells into megakaryocyte progenitor cells and megakaryocyte cells in vitro, which can solve the problems of low expression level and cumbersome methods.

Active Publication Date: 2010-10-20
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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Problems solved by technology

However, the method introduced in this literature is relatively cumbersome, and CD41 + Cell percentage 16.68±1.27%, 14 days CD41 + The cell expression level is 24.41±1.90%, showing that the expression level is not high

Method used

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  • Method for inducing megakaryoblast and megakaryocyte in vitro
  • Method for inducing megakaryoblast and megakaryocyte in vitro
  • Method for inducing megakaryoblast and megakaryocyte in vitro

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Abstract

The invention discloses a method for inducing megakaryoblast and megakaryocyte in vitro and a special culture medium thereof. The special culture medium is a serum free medium containing 50ng / mL of SCF, 50ng / mL of TPO, 20ng / mL of IL-3 and 50ng / mL of IL-6. The method comprises the following steps of: 1) separating single karyocyte from umbilical cord blood by using the conventional Ficoll density gradient centrifugation method, wherein red blood cells in the umbilical cord blood are settled by 6 percent hydroxyethyl starch; and 2) culturing the single karyocyte in vitro for 4 to 14 days by using the special culture medium at 37 DEG C in the presence of 5 percent CO2 to obtain a mixture of the megakaryoblast and the megakaryocyte. The invention provides a source for supplementing the megakaryoblast and the megakaryocyte, and has the advantages of simple operation, low cost, high differentiation efficiency and the like. The method is about to play an important role in the field of medicaments, and has wide application prospect.

Description

technical field The invention relates to an in vitro induction method of mononuclear cells in the field of biotechnology, in particular to a method for inducing differentiation of mononuclear cells into megakaryotic progenitor cells and megakaryocytes in vitro. Background technique Tumor is a new organism formed by the body under the action of various carcinogenic factors, a certain cell in the local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal cell clonal proliferation. Tumors can be divided into benign and malignant. Malignant tumors are one of the major diseases that seriously endanger human health. The conventional treatment is still surgical treatment combined with radiotherapy and chemotherapy. After patients receive high-dose radiotherapy and chemotherapy, the hematopoietic function of the bone marrow is low, and thrombocytopenia often occurs, and in severe cases, the patient will die of hemorrhage. Platelet transfusion i...

Claims

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Application Information

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IPC IPC(8): C12N5/0786C12N5/078
CPCC12N2501/125C12N2501/145C12N5/0644C12N2501/2306C12N2500/90C12N2501/2303
Inventor 裴雪涛陈琳师伟刘大庆岳文吕洋李艳华谢小燕主鸿鹄
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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