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Marine fungi aspergillus unguis strain, active extract thereof and preparation method and use of active extract thereof and active components thereof

A technology of marine fungi and active components, which is applied in the field of antibacterial and fungal drugs, and can solve the problem of antibiotics losing their efficacy

Inactive Publication Date: 2010-10-13
DALIAN JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, in clinical treatment, it is found that the drug resistance of pathogenic microorganisms is becoming stronger and stronger, especially methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (commonly known as Pseudomonas aeruginosa) and Candida albicans (commonly known as albicans) are becoming more and more serious, and many traditional antibiotics have basically lost their efficacy. Therefore, it is necessary to find new types of antibiotics and antibiotic-producing bacteria to meet this challenge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Seed solid medium:

[0036] Sucrose 20.0g;

[0037] 500ml potato juice;

[0038] Agar 15.0g;

[0039] 4% semi-artificial sea water 500ml;

[0040] natural pH.

[0041] Liquid medium:

[0042] Sucrose 20.0g;

[0043] 500ml potato juice;

[0044] 4% semi-artificial sea water 500ml;

[0045] natural pH.

[0046] Potato juice: take fresh potatoes and cut into 1cm 3 For every 200g of potato pieces, add 500ml of distilled water and boil for 20min, filter through double-layered gauze, and distill the volume to 500ml with distilled water.

[0047] 4% semi-artificial seawater: 40g coarse natural sea salt, dissolved in 1000ml distilled water, obtained by filtration.

[0048] A. Seed culture: Transfer the strains from the slope to a sterile solid medium plate, and place the culture plate in a constant temperature incubator for cultivation at 28°C. The culture time is 5 days;

[0049] B. Expanded cultivation: Fill the liquid culture medium into a breathable antibacteria...

Embodiment 2

[0053] Seed solid medium:

[0054] Sucrose 20.0g;

[0055] 500ml potato juice;

[0056] Artificial sea water 500ml;

[0057] Agar 15.0g;

[0058] natural pH.

[0059] Liquid medium:

[0060] Sucrose 20.0g;

[0061] 500ml potato juice;

[0062] Artificial sea water 500ml;

[0063] natural pH.

[0064] Potato juice: take fresh potatoes and cut into 1cm 3 For every 200g of potato pieces, add 500ml of distilled water and boil for 20min, filter through double-layered gauze, and distill the volume to 500ml with distilled water.

[0065] Artificial seawater: NaCl 25.0g, NaCl 2 SO 4 4.0g, KCl 0.70g, NaHCO 3 0.20g, KBr 0.10g, H 3 BO 3 0.03g, NaF 0.003g, MgCl 2 5.04g, CaCl 2 1.11g, SrCl 2 0.014g, dissolved in 1000ml distilled water, obtained by filtration.

[0066] A. Seed culture: Transfer the strains from the slope to a sterile solid medium plate, and place the culture plate in a constant temperature incubator for cultivation at 28°C. The culture time is 5 day...

Embodiment 3

[0070] Embodiment 3. active extract and active component activity determination result

[0071] Micro broth dilution method (National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that growaerobically, 5th ed. Approved standard M7-A6.; National Committee for Clinical Laboratory Standards. 1997. Reference th dilution method for bacterial antifungalsusceptibility testing of yeasts: approved standard M27-A, 7th ed.NCCLS, Wayne, Pa.) Determination of MIC values ​​against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Candida albicans , confirming that the total crude extract obtained by combining the ethyl acetate extract of the fermentation broth and the methanol extract of the mycelia of the culture broth has anti-MRSA Staphylococcus aureus, Pseudomonas aeruginosa and weak anti-Candida albicans activity, Its MIC value is respectively 8μg / mL, 4μg / mL and> 128μg / mL (the inhibit...

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PUM

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Abstract

The invention belongs to a stain of biological field and relates to marine fungi aspergillus unguis, active extract thereof and a preparation method and the use of the active extract thereof and active components thereof. A stain of marine fungi strain DLEP2008001 separated from Dalian sea area is appraised as aspergillus unguis. After the bacterial stain is fermented in a shake flask, the ethyl acetate extract of the fermentation liquor of the bacterial strain is combined with the alcohol extract of mycelia to form the total coarse extract having methyl penicillin-resistant staphylococcus aureus, pseudomonas aeruginosa and Candida albicans resistance activities; and the extract is subjected to silica gel column chromatography, silica gel thin-layer chromatography and reversed-phase high-efficiency liquid phase separation to obtain two active ingredients, namely a white powder component 1 and a component 2. The extract has remarkable antibacterial activities for the methyl penicillin-resistant staphylococcus aureus, the pseudomonas aeruginosa and the Candida albicans and has potential use as an antibacterial agent.

Description

technical field [0001] The present invention relates to a strain of marine fungus Aspergillus clawus DLEP2008001, especially the preparation method of the active extract and active components of the strain culture and the application of the extract and components in antibacterial and fungal drugs. Background technique [0002] At present, in clinical treatment, it is found that the drug resistance of pathogenic microorganisms is becoming stronger and stronger, especially methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (commonly known as Pseudomonas aeruginosa) and Candida albicans (commonly known as The infection caused by Candida albicans is becoming more and more serious, and many traditional antibiotics have basically lost their efficacy. Therefore, it is necessary to find new antibiotics and antibiotic-producing bacteria to meet this challenge. Research in the past half century has found that the potential of terrestrial microorganisms as a sou...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A61K36/06A61P31/04A61P31/10C12R1/66
Inventor 张翼穆军顾晓洁冯妍徐亚周李赫男陈明谷朋娟
Owner DALIAN JIAOTONG UNIVERSITY
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