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Fungus strain irpex iacteus for producing laccase, and culturing method and application thereof

A technology of white rake-tooth bacterium and a cultivation method, which is applied in the field of bioengineering, can solve the problems of long fermentation period, etc., and achieve the effects of shortening the fermentation period, low cultivation cost, and favorable comprehensive utilization

Inactive Publication Date: 2010-10-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid fermentation time is concentrated in about one month, the fermentation period is long, and most of the trace elements and inducers are added during the fermentation process

Method used

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  • Fungus strain irpex iacteus for producing laccase, and culturing method and application thereof
  • Fungus strain irpex iacteus for producing laccase, and culturing method and application thereof
  • Fungus strain irpex iacteus for producing laccase, and culturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and screening of fast-growing laccase-producing strains:

[0030] 1. Medium:

[0031] Separating medium: add 1L water to 20% (200g) bran, 30min after boiling, filter with 6 layers of gauze; weigh 2% (20g) agar strips into a 1000ml Erlenmeyer flask; fill the filtered bran liquid In this Erlenmeyer flask, add water to dilute the volume to 1000ml; sterilize the bran juice medium and the washed petri dish at 121°C for 20 minutes, add penicillin and streptomycin to 200mg / L, pour the plate; set aside.

[0032] High-throughput rapid growth laccase production selective medium: add 1L water to 10% (100g) bran, 30min after boiling, filter with 6 layers of gauze; weigh 2% (20g) agar strips into a 1000ml Erlenmeyer flask; Fill the filtered bran liquid into this triangular flask, add water to make the volume to 1000ml; sterilize the bran juice medium and the washed petri dish at 121℃ for 20 minutes, add guaiacol to 200mg / L, pour the plate spare.

[0033] Liquid selecti...

Embodiment 2

[0041] Example 2 Identification of the fast-growing laccase-producing strain H. albicans

[0042] 1. Morphological and physiological and biochemical identification

[0043] First, compare the collected fruit bodies or the fruit bodies produced by culture and the spore morphology with the fungal taxonomy manual "Fungi Identification Manual" (by Wei Jingchao) for preliminary identification of the strain. It is porous in the early stages of development. The upper surface is white to cream or light yellow, with dense bristles, without ring bands, with shallow grooves, and the edges are the same color. The surface of the orifice is white to cream. The orifice has edges and corners, one every millimeter near the edge. The wall of the orifice is thin, and it cracks into rake teeth in the early stage. The flesh is white to light in color, soft fiber, without ring bands. Basidiomycetes is one year old, flat to reverse curl, cap 8~15×9~20mm, thickness 1.5~3mm, white surface, with long sle...

Embodiment 3

[0046] Example 3 Liquid fermentation measurement of laccase

[0047] The synthetic medium lacks some growth factors, and most of the bacteria grow slowly on the synthetic medium. Harrowing tooth fungus grows rapidly on the above-mentioned medium, and the growth and laccase production reach the maximum on the eighth day. The enzyme production can be increased after adding veratrol as an inducer, indicating that the strain can produce both constitutive laccase and inducible laccase. Selective experiments were carried out on the carbon source and nitrogen source of the medium. Carbon sources include glucose, maltose, sodium oxalate, and citric acid; nitrogen sources include ammonium sulfate, ammonia chloride, urea, sodium nitrate, and diammonium phosphate. The experiment found that the best carbon source and best nitrogen source for this liquid medium are respectively It is glucose and diammonium bisulfate. After optimizing the culture conditions, an optimized enzyme-producing syn...

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Abstract

The invention relates to a novel fast-growing fungus strain irpex iacteus for producing laccase efficiently, and a culturing method and application thereof, and belongs to the technical field of biological engineering. The irpex iacteus sdu-5 is preserved in the China General Microbiological Culture Collection Center on December 28, 2009, and the preservation number is CGMCC NO.3548. The strain can produce the laccase economically and efficiently under the condition that an inducer is added into a liquid nutrient medium or no inducer is added into a solid fermentation nutrient medium.

Description

Technical field [0001] The invention relates to a new fast-growing high-efficiency laccase-producing fungal strain H. albicans and its cultivation method and application, belonging to the technical field of bioengineering. Background technique [0002] Laccase is a protein that was first used by humans. Laccase is a glycoprotein. The molecular weight of the monomer ranges from 50kD to 110kD, and the sugar content ranges from 10% to 45% of the molecular weight. The laccases found so far are mainly tetramers, in addition to dimers and monomers, and there are large differences in sequence homology. The convergent evolution of proteins in the biological world and the wide substrate specificity of laccases make the classification of laccases not very clear. There are a large number of proteins with similar activities of laccases. Laccase has a typical structure of four copper ions so that people can define the laccase and determine the purity of the laccase by its spectroscopic abso...

Claims

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Application Information

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IPC IPC(8): A01G1/04C12N9/02
Inventor 黄峰张海波张应龙李夏
Owner SHANDONG UNIV
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