Method for directly inducing and quickly propagating bog bilberry root nodule in test tube

Abstract

The invention relates to a method for plant propagation, in particular to a method for directly inducing and quickly propagating a bog bilberry root nodule in a test tube. The method is characterized by comprising the following steps of: (1) selecting a tiller bud of a bog bilberry root as an explant, wherein a proper nutrient medium for inducing a bud root nodule is 1 / 4MS+IAA0.80mg.L-1+IBA0.04mg.L-1+GA30.15mg.L-1; and (2) selecting a stem node growing the root nodule already of a reproducing plant as a material to perform quick propagation in the test tube by a propagation method, cutting a seedling which contains the root nodule in the test tube from the upper part of the root and leaving 1 or 2 leaves on the cut part, cutting the cut twig into sections which have a leaf respectively, grafting the sections into the optimized root nodule induction nutrient medium to allow auxiliary buds to sprout and grow and culturing the root nodule to regenerate the root nodule. The method shortens a successive multiplication cycle, improves propagation folds, is simple, economical, practical and strongly operable, achieves the quick propagation aim and can be used for the industrial breeding of the bog bilberry having the root nodule.

Description

technical field [0001] The invention relates to a kind of plant propagation, that is, the method of direct induction and rapid propagation in test tube of Vaccinium vaccinium root nodules. Background technique [0002] In the prior art, Vaccinium uliginosum Linn., also known as Dusi, Dianguo, Diguo, Dragon Fruit, Narisu (Mongolian language). Vaccinium dusi is a small deciduous shrub of the Rhododendronaceae Vaccinium genus. It is designated as a provincial-level key protected plant in the "Interim Regulations on the Protection and Management of Wild Animals and Plants of Jilin Province". It is a wild ornamental plant and a rare and endangered medicinal plant. Its main functions are heat-clearing and astringent. The fruit can be eaten raw, sweet and sour, and rich in juice. It is used as a raw material for fruit wine, fruit vinegar, jam, fruit juice and extraction of natural food coloring. It is designated as one of the five health foods by the International Food and Agricul...

AI Technical Summary

Problems solved by technology

In this study, the direct induction of root nodules and the high-efficiency and rapid propagation of root-nodulated seedlings in Vaccinium baicalensis in test tubes have not been reported at home and abroad so far.

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