In-vitro direct induction and mycorrhization germchit rapid propagation method of rhododendron mycorrhiza

Abstract

The invention relates to a plant propagation technology, and particularly relates to an in-vitro direct induction and mycorrhization germchit rapid propagation method of rhododendron mycorrhiza. The method comprises the following steps of: 1, treating an explant material, to be specific, picking a tender shoot newly germinated by rhododendron, which is close to the root part, in the middle ten days of August, disinfecting and cutting into a 1-2-leaf section to be used as the explant for later use; 2, enabling a culture medium induced by rhododendron mycorrhiza to be a basic culture medium and KT0.50mg.L <-1> and IAA0.02mg.L<-1> and GA30.70mg.L<-1> to obtain the explant; 3, culturing the explant on the basic culture medium and ZT2.20mg.L<-1> into a small and tender axillary bud, after the axillary bud grows to 1.00-2.00cm, cutting the axillary bud from the axillae and grafting to the basic culture medium and KT0.50mg.L<-1> and IAA0.02mg.L<-1> and GA30.70mg.L<-1>, culturing for 60d, counting and figuring out the induction rate of a mycorrhized seedling; and 4, rapidly propagating mycorrhized plants. A rhododendron mycorrhiza seedling rapid propagation system is established, and the basic guarantee is provided for growth and development of the rhododendron and the improvement of the quality. The in-vitro direct induction and mycorrhization germchit rapid propagation method of the rhododendron mycorrhiza can be directly applied to factorized production of rhododendron mycorrhiza seedlings.

Description

technical field [0001] The invention relates to a plant propagation technology, namely a method for direct induction and mycorrhizal seedling rapid propagation in test tube of rhododendron mycorrhizae. Background technique [0002] In the prior art, Rhododendron roots have no root hairs, and their absorptive capacity is much smaller than that of roots with root hairs, but under natural conditions, almost all rhododendron fine roots have EM (Eriocdimyochrriaz, EM) endomycorrhizal fungus parasitism , so as to overcome the difficulty in absorbing water and nutrients caused by rhododendrons without root hairs, improve the nutritional status of plants, regulate the metabolic activity of the host, enhance the stress resistance of plants, increase the yield of rhododendrons, speed up the survival rate of transplanted seedlings, and save Agricultural production costs, increase economic output and benefits. Therefore, combining rhododendron tissue culture technology with mycorrhizal...

AI Technical Summary

Problems solved by technology

The establishment of Rhododendron mycorrhizae is mostly completed by multi-step methods such as seedling propagation followed by mycorrhizal fungal infection. These methods have problems such as complicated steps and operations, and infection rate.

The report of the direct induction of mycorrhizae in the test tube of Rhododendron and the high-efficiency and rapid propagation of mycorrhizal seedlings carried out by the present invention have not been seen so far at home and abroad

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