Method for preparing protein chip by in-situ synthesis

Abstract

In order to solve the problem, which troubles academic circles for a long time, about how to permanently bond protein molecules to the metal actively and fixe the protein molecules on the metallic surface of any detector or any independent addressing electrode in place at one step, the invention provides a method for preparing a protein chip. The method comprises the following steps of: by adopting an in-situ electrochemical combination synthesis method and independent addressing microelectrode array technique, making an aniline derivative monomer containing bioactive units and anilines in solution copolymerized on an electrode in a mode of active free radical polymerization, generating an electrode coverings which is bioactive and electrically conductive on the electrode in situ at one step, and under bionic conditions, making avidins connected with various proteins tightly bonded with the e electrode coverings to prepare the protein chip. The method has the advantages of laying a foundation for an unmarked analysis method, truly making mass production of protein chips possible, providing the possibility for the automatic production of the protein chips at the operation level forthe first time, along with high reproducibility.

Description

A method for preparing protein chips by in situ synthesis technical field The invention relates to a method for preparing a protein chip, in particular to a microelectrode array technology using an in-situ electrochemical combinatorial synthesis (combinatorial synthesis) method and independent addressing to make aniline derivatives with biologically active units single The body is copolymerized with aniline in the solution on the electrode by living free radical polymerization, and a biologically active and conductive electrode covering is directly formed on the electrode in situ in one step, and the avidin with a variety of proteins is connected to it under biomimetic conditions. A method for making a protein chip by strong binding. Background technique Biochip technology is a high-tech developed rapidly in the field of life science since the 1990s. It mainly refers to the construction of a miniature biochemical analysis system on the surface of a solid chip through micr...

AI Technical Summary

Problems solved by technology

The first is the cost issue. The current protein chip production process is quite cumbersome and complicated, and the signal detection also requires special and expensive equipment (such as seldi-tof-ms); Small changes may cause differences in the final results, and the experimental conditions are not easy to control, making the repeatability of the experimental results relatively insufficient

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