Method for in-vitro rapid propagation of clivia miniata by using mature embryos as explants

Abstract

The invention belongs to a plant propagation method, and particularly relates to a method for the asexual rapid propagation of flowers. The method utilizes the technique such as detoxification, inoculation and callus induction of explants, induction of adventitious shoots, root induction and transplant of regeneration plants for the asexual rapid propagation of clivia miniata. When the method is used, the selection of raw materials is not limited by seasons, the method is suitable for agrobacterium-mediated transgenes, and the silence of the transgenes is overcome to some extent; the mature embryos from ten plants of mature clivia miniata are used as the explants, and the dedifferentiation rate and the differentiation rate both reach 70 to 100 percent; the regeneration coefficient is high, and an original callus tissues can grow into hundreds of cluster buds by repeated sub-culture; the rooting rate of the cluster buds is 100 percent, and the transplanting survival rate is 100 percent. When the method is used, the clivia miniata in different varieties can mass propagate the plants which have the same genetic background and smooth appearances in a shorter time; and after the plants are transplanted, the growing state is good, the cost for the average individual-plant regeneration seedling is low, and the method is suitable for industrialized production.

Description

technical field [0001] The invention belongs to a plant propagation method, in particular to an asexual rapid propagation method of flowers. Background technique [0002] Plant tissue culture is plant aseptic culture technology, which is based on the totipotency of plant cells, using isolated plant organs (such as roots, stems, leaves, stem tips, flowers, fruits, etc.), tissues (such as cambium, epidermis, Cortex, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts, under sterile and suitable artificial medium and artificial conditions such as light and temperature, induce callus, adventitious buds, Adventitious roots, techniques for forming whole plants or producing other products of economic value. The regenerated plants produced by plant tissue culture can basically maintain the excellent traits of the parents (explant source plants) except for extremely low somaclonal variation. Since the invention of plant tissue culture te...

AI Technical Summary

Problems solved by technology

Referring to the relevant international and domestic literature, we know that although the previous Clivia tissue culture experiments have been initially successful, the dedifferentiation rate and regeneration rate are low, and an average of one explant can regenerate a few tenths to two tenths of seedlings depending on the variety.

This limits the application of Clivia tissue culture technology in actual production