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Method for extracting oat beta-glucan

A technology of glucan and oatmeal is applied in the directions of pharmaceutical formulations, preparations for skin care, and medical preparations containing active ingredients, which can solve the problems of high production temperature, high cost, and difficult removal, and achieve high safety and reduce Fine lines, improve facial redness

Active Publication Date: 2010-08-11
SHANGHAI SHENG WEI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all require the introduction of organic solvents, which are costly and difficult to remove, and the production temperature is high, which is not good for energy saving and environmental protection.

Method used

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  • Method for extracting oat beta-glucan
  • Method for extracting oat beta-glucan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Choose oat bran with a particle size of 20 mesh. The starch granules or powder and floating dust on the surface of the oat bran were removed by a starch sieving machine (Xinxiang Sanhe Vibration Equipment Co., Ltd.) using a 60-mesh sieve. Oat bran was stirred with water at 50° C., the weight ratio of oat bran to water was 1:20, pH was adjusted to 8, and stirred for 1 hour. After centrifugation at 5000 rpm, the filtrate was collected, cooled to 30° C., adjusted to pH 4.5, stirred and allowed to stand for 1 hour. The filtrate obtained by centrifugation at 5000rpm was added with low-temperature α-amylase (purchased from Sigma, A-3176, Type VI-B, enzyme activity 24000U / g), and the dosage was 0.42g low-temperature α-amylase / 100g oat bran. At 40° C., pH value 6, treated for 1 hour, filtered with diatomaceous earth to obtain a clear liquid. Filtrate with an ultrafiltration membrane with a molecular weight cut-off of 100,000 Da, collect the retentate, obtain oat β-glucan solu...

Embodiment 2

[0027] Choose oat bran with a particle size of 60 mesh. The starch granules or powder and floating dust on the surface of the oat bran are removed by using a 80-mesh sieve through a starch screening machine. The oat bran was stirred with water at 40° C., the weight ratio of the oat bran to water was 1:30, the pH was adjusted to 7, and the mixture was stirred for 5 hours. Filter through a 100-mesh filter cloth, collect the filtrate, cool to 10°C, adjust the pH to 5.5, and let stand for 3 hours after stirring. Centrifuge at 5000rpm to obtain filtrate, add low-temperature α-amylase (enzyme activity 24000U / g), the dosage is 1.25g low-temperature α-amylase / 100g oat bran. At 45°C, the pH value was 6.5, treated for 2 hours, and filtered through a diatomaceous earth plate to obtain a clear liquid. Filtrate with an ultrafiltration membrane with a molecular weight cut-off of 50,000 Da, collect the retentate, obtain oat β-glucan solution, and freeze-dry. The extraction rate is 78%, an...

Embodiment 3

[0029] Choose oat bran with a particle size of 40 mesh. Use a 60-mesh sieve through a starch sieving machine to remove starch granules or powder and floating dust on the surface of the oat bran. Oat bran was stirred with water at 45° C., the weight ratio of oat bran to water was 1:25, pH was adjusted to 7.5, and stirred for 2 hours. After centrifugation at 10,000 rpm, the filtrate was collected, cooled to 20° C., adjusted to pH 5.0, and allowed to stand for 0.5 hours after stirring. Centrifuge at 5000rpm to obtain filtrate, add low-temperature α-amylase (enzyme activity 24000U / g), the dosage is 0.85g low-temperature α-amylase / 100g oat bran. At 35° C., with a pH value of 5.5, it was treated for 1.5 hours, and filtered through a diatomaceous earth plate to obtain a clear liquid. Filter with an ultrafiltration membrane with a molecular weight cut-off of 80,000 Da, collect the retentate, obtain oat β-glucan solution, and freeze-dry. The extraction rate is 82%, and the purity is...

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Abstract

The invention discloses a method for extracting oat beta-glucan, which comprises the following steps: 1) removing starch and floating dust on the surface of oat bran; 2) adding water into the oat bran at 40-50 DEG C, regulating the pH to 7-8, and stirring for 1-5 hours; 3) collecting the filtered liquid after separation, cooling to 10-30 DEG C, regulating the pH to 4.5-5.0, stirring, and then standing for 0.5-3 hours; 4) centrifuging and separating to obtain the filtrate, adding low-temperature alpha-amylase, treating for 1-2 hours under the conditions that the temperature is 35-45 DEG C and the pH value is 5.5-6.5, and filtering with a kieselguhr sheet frame to obtain supernatant liquid; and 5) filtering with a ultrafiltration membrane of which the molecular weight cut-off is 50,000-100,000Da to obtain an oat beta-glucan solution. The organic solvents are not used in the production process of the oat beta-glucan of the invention, operating temperature is not higher than 50 DEG C, and has the advantages of energy saving, environmental protection and high safety. The invention also discovers the application of the oat beta-glucan in improving flushed face and reducing fine wrinkles.

Description

technical field [0001] The invention belongs to the field of plant extracts, in particular to a method for extracting oat beta-glucan. Background technique [0002] Oat β-glucan is extracted from oat bran, and the added value of the product increases greatly after extraction. Oat β-glucan is a viscous polyglucan linked by β-1,3-, β-1,4-glycosidic bonds, which is mainly concentrated in the aleurone layer of oat bran. Oat β-glucan has multiple functions, such as lowering blood lipids, controlling blood sugar, enhancing immunity, preventing constipation, improving intestinal microenvironment, etc., and is widely used in the fields of medicine and health food. [0003] At present, the preparation of oat β-glucan is generally carried out by solvent extraction or mild alkali extraction. These methods all need to introduce organic solvents, which are costly and difficult to remove, and the production temperature is relatively high, which is unfavorable to energy saving and enviro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12S3/02A61K8/73A61K31/716A61P17/00A61Q19/08
CPCY02P20/10
Inventor 李成亮翟春涛廖立平孙常磊
Owner SHANGHAI SHENG WEI BIOTECHNOLOGY CO LTD
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