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Fibrin sealant and preparation method thereof

A technology of fibrin and fibrinogen, applied in the direction of peptide/protein components, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of poor feasibility, few sources, long time, etc., and achieve good wound healing and scar Small, easy to store effects

Active Publication Date: 2010-08-11
BEIJING SAISHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of human thrombin can solve some problems, the possibility of using human thrombin virus infection still exists, and the source is few and the cost is high
Using human thrombin to prepare fibrin glue, the operation is complicated, the time is long and the feasibility is poor

Method used

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  • Fibrin sealant and preparation method thereof
  • Fibrin sealant and preparation method thereof

Examples

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Embodiment 1

[0040] A. Preparation of fibrinogen and its buffer solution: Melt and cool porcine whole plasma at 37°C to below 10°C, slowly add 50% ethanol at a volume percentage of pre-cooled -20°C and keep stirring until the final concentration is 10% ethanol, as 12 hours of precipitation treatment, centrifuged at 5000 rpm for 30 minutes. The precipitate was then dissolved in neutral Tri / citrate-lysine buffer solution with a final protein concentration of about 1.5% (weight percent). Then pre-cooled 50% ethanol for 10-12 hours at 4°C for the second 10% ethanol precipitation. Centrifuge at 5000 rpm for 30 minutes to dissolve the precipitate to 40mg / ml, then sterilize and filter, and divide into 5ml bottles for lyophilization. The fibrinogen solution is a buffer solution containing 0.02M pH7.4 sodium citrate.

[0041] Type B thrombin preparation and its buffer:

[0042] Method 1: Weigh 10 g of Agkistrodon halys venom (purchased from Liaoning Qingyuan Viper Breeding Factory), extract with...

Embodiment 2

[0048] The prepared fibrinogen was dissolved to 50mg / ml for later use, and the thrombin-like enzyme was prepared at 100 international units per milliliter. Take 2 milliliters of fibrinogen solution, pour it into the mould, add 60 IU of glycerol-like thrombin containing 1% and mix well, make it react completely, form a colloid, and freeze-dry.

Embodiment 3

[0050] The fibrinogen and thrombin-like solution are mixed according to the ratio of 100 mg of fibrinogen to 10 international units of thrombin-like, freeze-dried after fully reacting, crushed into fine powder, and packed into capsules.

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Abstract

The invention provides a fibrin sealant which comprises a fibrin polymer and a thrombin-like enzyme, wherein a ratio of the thrombin-like enzyme to the fibrin polymer is 0.01 to 100 IU:1 mg. The fibrin sealant can be used on the aspects of hemostasis, healing promotion, defect enclosing, bonding prevention, tendon suture fixation tendon injury, molding repair of bony defect, regeneration of skin tissue, carrier slow release and the like.

Description

technical field [0001] The invention relates to a fibrin glue that can stop bleeding, can be used as a culture medium, can be used as a drug carrier, and can replace skin, bone, cartilage, nerve and other tissues and its preparation method, more specifically, a fibrin sealant and its preparation method. Background technique [0002] Fibrin sealant (Fibrin Sealant, referred to as FS), its mechanism of action is to simulate the final stage of blood coagulation, under the action of thrombin and calcium ions, convert fibrinogen into fibrin monomer, and further polymerize into fibrin Network, and under the action of factor XIII, form a cross-linked and stable fibrin network, thereby exerting its hemostatic effect. In recent decades, the use of FS is no longer limited to the four major functions of hemostasis, promotion of healing, closure of defects, and prevention of adhesion. Instead, it can be used to suture muscle damage in the tendon with the help of the adhesive properties...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61K9/00A61P7/04A61K38/36A61K38/18A61K33/06
Inventor 马骉孔双泉宋梦薇王靖
Owner BEIJING SAISHENG PHARMA
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