Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extracts with liver-X-receptor modulators, compounds and their use especially in weight control

A compound, the technology of -ra, is applied in the field of one or more natural compounds alone or as an additive, as an active ingredient, and can solve problems such as unreasonable effects

Inactive Publication Date: 2010-08-04
INTERMED DISCOVERY
View PDF12 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even more surprisingly, these concentrated extracts did not need to contain gomisin G or benzoylgomisin Q as they showed activity (albeit weak) on NPFF (see above), whereas as in the examples As shown in , they were not expected to cross the blood / brain barrier making their effect on NPFF unreasonable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extracts with liver-X-receptor modulators, compounds and their use especially in weight control
  • Extracts with liver-X-receptor modulators, compounds and their use especially in weight control
  • Extracts with liver-X-receptor modulators, compounds and their use especially in weight control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0176] Embodiment 1: Schisandra methanol (1), the pure change:

[0177] 1 kg of dried Schisandra fruit (Galke, Gittelde / Harz, Germany) was extracted by sonication at room temperature for 30 minutes using 1800 ml of 95% ethanol and finally crushed for 10 minutes using a high-speed disperser ultra-turrax (Janke & Kunkel, Staufen, Germany). After vacuum filtration, the remaining fruit material was extracted a second time for 30 minutes using 700 ml of 95% ethanol. The combined extract solvents were evaporated under reduced pressure. The remaining aqueous phase (about 250 ml) was extracted 3 more times with 250 ml ethyl acetate. The collected ethyl acetate extracts were evaporated to dryness under reduced pressure to obtain an extract hereinafter referred to as crude extract.

[0178] For further enrichment of pure compounds, the crude extract was subjected to a first purification step using a Nucleodur 100-5C18ec column (Macherey & Nagel, Düren, Germany) (250x20 mm) with li...

Embodiment 2

[0199] Example 2: Further enriched extract from Schisandra chinensis

[0200]The crude extract (see Example 1) was subjected to a chromatographic concentration step using a Nucleodur 100-5C18ec column (Macherey & Nagel, Düren, Germany) (130x40 mm), where a linear water (solvent A) / acetonitrile (solvent B) gradient was applied (two The solvents all contained 0.1% trifluoroacetic acid), starting from 30% acetonitrile to 100% acetonitrile in 60 minutes, followed by an isocratic period of 20 minutes. Fractions were collected every minute using a flow rate of 20ml / min. Signals were detected at 210 nm and 254 nm using a UV / VIS-155 detector (Gilson, Langenfeld, Germany). Fractions containing deoxyschisandra methanol, gomisin N and schisandrin (retention time 32-62 min) were collected and combined. The method can directly concentrate LXR active lignans and next-level LXR active compounds.

[0201] Peaks measured using various detection systems in HPLC such as figure 1 shown in . ...

Embodiment 3

[0240] Embodiment 3: Experiment in mice

[0241] The extract from Example 2 was applied as a supplement to mouse chow, and changes in body weight and body fat compared to a control group not subjected to such supplementation were measured.

[0242] By feeding a standard diet (Atromin 1324, AtrominSpezialfutterwerke GmbH, Lage, Germany) 8-week-old male mice [strain C57BL / 6 (Harlan Winkelmann GmbH, Borchen, Germany)] were acclimatized for 2 weeks.

[0243] Feeding experiment:

[0244] cc: Long-term balanced diet: after acclimatization standard food enriched with 1mg extract / 1g dry food.

[0245] cf: balanced / high-fat meal: enriched with 1mg extract / 1g dry food and 12% palm fat (Peter KGaA, Elmshorn) after acclimation to standard food.

[0246] Mice were weighed weekly (accuracy ±0.1 g) and thereafter scanned using DEXA under isoflurane (2-chloro-2-(difluoromethoxy)-1,1,1-trifluoro-ethane) anesthesia Body composition was analyzed using a PIXImus2 scanner, software version ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the use, or methods (especially with regard to animals, especially human, that are in need of such treatment) comprising the use, of an extract and / or one or more natural compounds from plants or parts of plants, respectively, from a genus selected from the group consisting of Schisandra,Illicium, Kadsura, Steganotaenia and Magnolia, alone or as supplement, as active ingredient in the regulation of body weight and / or fat loss and / or for the management of obesity, either in humans or in animals, to the use of said extract and / or natural compound(s) or mixtures in the manufacture of a pharmaceutical or nutraceutical formulation for the regulation of body weight and / or fat loss and / or for the management of obesity either in humans or in animals. The above extract and / or compound(s) can further be used to reduce one or more adverse metabolic parameters in a subject. The invention relates also to said extract and / or compound(s) for use in the treatment or in the preparation of a medicament for the treatment of obesity, as well as their preparation. It also relates to pharmaceutical or nutraceutical formulations comprising said extract and / or natural compound(s) which are useful in the regulation of body weight and / or fat loss and / or for the management of obesity.

Description

technical field [0001] The present invention relates to extracts and / or one or more natural Use of a compound alone or as an additive (supplement), as an active ingredient, in regulating body weight and / or reducing fat and / or controlling obesity in humans or animals or methods comprising the use (especially in animals in need of such treatment, Especially for humans), relates to the use of said extract and / or natural compound or mixture in the preparation of medicaments or nutritional products for regulating human or animal body weight and / or reducing fat and / or controlling obesity. The above extracts and / or compounds can be further used for anti-inflammation. The present invention also relates to the application and preparation of the extract and / or compound in treating obesity or preparing medicine for treating obesity. The invention also relates to pharmaceutical or nutraceutical preparations comprising said extracts and / or natural compounds for regulating body weight and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/085A61K31/215A61K31/343A61K36/79A61K36/57A61P3/04A61P3/06
CPCA61K31/215A61K36/57A61K31/222A61K31/343A61K31/085A61K31/36A61K36/79A61K31/09A61P3/00A61P3/10A61P3/04A61P3/06A61P43/00A61P9/10A61P9/12
Inventor T·格罗特E·勒默尔T·亨克尔
Owner INTERMED DISCOVERY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com