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Lactose diagnosis/measurement reagent (kit) and method for measuring lactose concentration

A reagent, lactose technology, applied in the field of medical/food testing and determination, can solve the problems of zinc deficiency, affecting iron and zinc absorption, increasing bone loss, etc.

Inactive Publication Date: 2010-08-04
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to foreign research, lactose intolerance can reduce calcium intake, reduce peak bone mass, increase bone loss and cause osteoporosis. Lactose intolerance can affect the absorption of iron and zinc, resulting in iron deficiency and zinc deficiency, and It can affect the brain development of children and is very harmful to human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The lactose diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0041] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0042] Stabilizer 500mmol / L

[0043] Reduced coenzyme 0.25mmol / L

[0044] Lactase 6000U / L

[0045] Glucose-6-phosphatase 8000U / L

[0046] Aldose-6-phosphate reductase 10000U / L

[0047] Phosphate 7mmol / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the lactose sample to be tested is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0050] After addi...

Embodiment 2

[0052] The lactose diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Reduced coenzyme 0.25mmol / L

[0057] Phosphate 7mmol / L

[0058] Reagent 2

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 500mmol / L

[0061] Lactase 6000U / L

[0062] Glucose-6-phosphatase 8000U / L

[0063] Aldose-6-phosphate reductase 10000U / L

[0064] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0065] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the tested lactose sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (d...

Embodiment 3

[0068] The lactose diagnosis / measurement reagent of this embodiment is three reagents, comprising:

[0069] Reagent 1

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 50mmol / L

[0072] Reduced coenzyme 0.25mmol / L

[0073] Phosphate 7mmol / L

[0074] Reagent 2

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 500mmol / L

[0077] Glucose-6-phosphatase 8000U / L

[0078] Aldose-6-phosphate reductase 10000U / L

[0079] Reagent 3

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Lactase 6000U / L

[0083] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0084] When measuring lactose concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength...

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Abstract

The invention relates to a lactose diagnosis / measurement reagent (kit) by using the technology of an enzyme colorimetric method and an enzyme coupling method, also relates to a method for measuring lactose concentration, and the composition and components of the reagent, and belongs to the technical field of medicine / food inspection and measurement. The reagent (kit) of the invention comprises the following components: buffer solution, NADH, phosphate group, lactase, glucose-6-phosphatase, aldose-6-phosphate reductase and a stabilizing agent. A sample and a reagent are mixed in a certain volume ratio to undergo a series of enzymatic reaction, the reactant is placed under an ultraviolet / visible light analytical instrument, and the rising degree of the absorbance of the reactant at the main wavelength of 340nm is measured, so that the lactose concentration is measured.

Description

technical field [0001] The invention relates to a lactose diagnosis / measurement reagent (box), and simultaneously the invention also relates to a method for measuring lactose concentration, which belongs to the technical field of medicine / food inspection and determination. Background technique [0002] Lactose is the main source of energy for infants. After entering the body, it is decomposed into glucose and galactose by the action of lactase in the small intestine. Galactose is an essential substance for infant brain development and is closely related to the rapid growth of infant brain. In order to prevent whey powder from being mixed with other reducing sugars such as glucose instead of lactose, the detection of lactose is very necessary. [0003] Milk is a nutritious food, but if it is not drunk properly, it will also have adverse effects on health. Many people have symptoms such as abdominal discomfort, bloating, excessive fart, abdominal pain and even diarrhea after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N35/00C12Q1/26C12Q1/54C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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