Optimum condition for proliferating Guangxi epidemically representative strains of infectious bursal disease virus (IBDV) in chicken embryo

A chicken infectious and bursal technology, applied in the field of biology, can solve the problems of decreased resistance, decreased immune response levels of various vaccines, decreased immune function, etc.

Inactive Publication Date: 2010-08-04
GUANGXI UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

It can not only cause the clinical onset of young chickens, but also reduce the immune function due to immunosuppression caused by subclinical infec

Method used

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Experimental program
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Embodiment Construction

[0017] The present invention will be further described below in conjunction with embodiment.

[0018] The optimal conditions for chicken embryo propagation of chicken infectious bursal virus Guangxi popular representative strain are as follows:

[0019] 1. Exploration of the optimal inoculation route of each virus strain and the virus multiplication yield of each part of chicken embryo

[0020] Select 8 well-developed 9-day-old chicken embryos per strain, 0.2 mL / embryo, and inoculate the chorioallantoic membrane and the allantoic cavity respectively. Collect dead embryos at 24h-96h and live embryos with IBDV characteristic lesions, harvest chorioallantoic membrane, embryo body and allantoic fluid respectively, cut them finely with scissors, grind them with a mortar, freeze and thaw three times, and centrifuge to get the supernatant. Packed in sterilized bottles respectively. Sterility checks were performed with blood agar plates, and hemagglutination tests were performed wit...

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Abstract

The invention discloses an optimum condition for proliferating Guangxi epidemically representative strains of infectious bursal disease virus (IBDV) in chicken embryos. The optimum condition is an orthogonal design combination of seed virus inoculation route, inoculated embryo day age, inoculation density, embryo gathering time and gathering part when the virus candidate strain i.e. Guangxi epidemically representative strains 040124, BH11 and TSC-2(9), which are designed by biostatistics software, are proliferated in chicken embryos, and an optimum production parameter is explored to supply useful data to the commercial production of vaccines. The invention has the advantages of exploring the optimum condition and supplying useful data for the commercial production of the vaccines.

Description

technical field [0001] The present invention belongs to the field of biology, and in particular relates to the optimal condition for chicken embryo multiplication of the popular representative strain of chicken infectious bursal virus in Guangxi. Background technique [0002] Infectious bursal disease (IBD) is an acute, highly contact, lymphocytic infection that mainly harms young chickens caused by infectious bursal disease virus (IBDV). sick. It can not only cause the clinical onset of young chickens, but also reduce the immune function due to immunosuppression caused by subclinical infection, which reduces the immune response level and resistance of various vaccines, resulting in the occurrence of various diseases. cause greater losses. Therefore, the effective prevention and control of IBD can not only avoid the direct loss caused by IBD itself, but also help the prevention and control of other diseases by avoiding the immune suppression caused by IBD. [0003] Vaccin...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12R1/93
Inventor 韦平吴俊姬
Owner GUANGXI UNIV
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