Method for culturing and establishing extracorporeal hypospadias model by utilizing genital tubercle organ

Abstract

The invention discloses a method for culturing and establishing an extracorporeal hypospadias model by utilizing a genital tubercle organ and the extracorporeal hypospadias model cultured by the method. The method uses estrogen to intervene genital tubercle cultured in vitro; the pathology expression of the model is the same as the expression of the animal model of mouse hypospadias which is recognized currently; the growth of the genital tubercle is restrained obviously; the whole urethra is still a solid sex cell cord, the tube of urethra is not obvious, epitheliogenesis is unhealthy, and the arrangement is disorder. The construction of the model is characterized by avoiding the problems of high cost of animal experiments, long experiment period and easily-influenced research results by the in-vivo nerve-internal secretion factors in the correlational research process of urethra development. The model has high molding success rate, good repeatability, and high application value and is worth popularizing.

Description

【Technical field】 [0001] The invention relates to an application of organ culture, in particular to establishing an in vitro hypospadias model by using genital tubercle organ culture. 【Background technique】 [0002] Hypospadias is a common congenital deformity. It is an abnormal opening of the urethra due to hypoplasia of the urethra. It can be located anywhere from the penis to the perineal urethra. Developmental abnormalities, etc., will have a certain degree of impact on the patient's physiology and psychology. [0003] The incidence rate of hypospadias in male newborns is 1 / 125-1 / 250, and the incidence rate in my country is 3 / 1000. In recent years, the incidence of hypospadias has doubled for unknown reasons, which has attracted the attention of foreign health institutions and experts, and the United States has initiated research in this area. In recent years, some scholars in our country have gradually begun to study the disease. At present, there are various methods...

AI Technical Summary

Problems solved by technology

The animal models of hypospadias reported in current studies lack standardization, and each study can only represent hypospadias under a specific condition, which makes it difficult for all studies to be compared horizontally under the same standard

[0005] In addition, in animal experiments, the development of the penis and urethra is hidden deep in the uterus, which is not easy to observe, and the complex endocrine environment existing in the body is also difficult to elucidate its mechanism

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