Method for inducing non-alcoholic fatty liver disease through fluorine intervention
A non-alcoholic fatty liver technology, applied in the field of non-alcoholic fatty liver model induced by fluoride intervention in mice, can solve the problems of streptozotocin toxicity, long modeling time, and high model cost, and shorten the modeling time. The effect of mold cycle, simple experiment and low cost
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Embodiment 1
[0019] (1) Twenty-four SPF grade male C57BL / 6 mice (4 weeks old) were fed freely with food and water for one week and then randomly divided into three groups, with 8 mice in each group. The first group was fed with normal diet (Normal Diet, ND group); the second group was fed with high-fat diet (High-fat Diet, HFD group); the third group was fed with high-fat diet, and the concentration of sodium fluoride added in drinking water was 110mg / L (HFD-F group);
[0020] (2) After 10 weeks of intervention, the mice were fasted for 12 hours, and the mice were sacrificed by pulling the neck through the heart. After centrifugation, the plasma was obtained for biochemical analysis. After the mouse liver was weighed and recorded, it was put into the fixative used to prepare slices;
[0021] (3) Physiological and biochemical indicators in mouse plasma such as TG, TC, LDL-C and HDL-C were determined according to the kit instructions;
[0022] (4) After weighing the liver tissue of the mou...
Embodiment 2
[0025] (1) To verify the mechanism of fluorine-induced non-alcoholic fatty liver, purchase 16 SPF grade male Tlr4 knockout C57BL / 10J mice (4 weeks old) and 16 C57BL / 10J wild-type control mice (4 weeks old ), were randomly divided into 4 groups, with 8 mice in each group, after a week of adaptation to feeding with free access to food and water. The first group of wild type fed high-fat diet group (HFD group); the second group fed with high-fat diet and added sodium fluoride concentration of 110mg / L in drinking water (HFD-F group); the third group was Tlr4 knockout Mice fed high-fat diet group (TLR4-HFD); the fourth group was Tlr4 knockout mice fed high-fat diet group, and added sodium fluoride concentration of 110mg / L in drinking water (TLR4-HFD-F group) ;
[0026] (2) After 8 weeks of intervention, after the mice were fasted for 12 hours, the mice were sacrificed by pulling the neck through the heart, and the plasma was obtained after centrifugation for biochemical analysis. ...
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