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Method for efficiently extracting polysaccharide and polyphenol from agaricus blazei

A polysaccharide and high-efficiency technology, applied in the direction of antineoplastic drugs, fermentation, medical raw materials derived from fungi, etc., can solve the problems of protein interference, low extraction rate and activity, waste, etc., and achieve mild conditions, high yield, and purity high effect

Inactive Publication Date: 2010-07-07
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a method for extracting polysaccharides from Agaricus blazei for the prior art, which cannot eliminate protein interference, low extraction rate and activity, and can not simultaneously extract polyphenols, resulting in waste. Simultaneous, high-efficiency, low-cost, and more thorough method for extracting polysaccharides and polyphenols by removing protein impurities

Method used

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  • Method for efficiently extracting polysaccharide and polyphenol from agaricus blazei
  • Method for efficiently extracting polysaccharide and polyphenol from agaricus blazei

Examples

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Embodiment 1

[0035] Embodiment 1: (method 1 for simultaneously efficiently extracting polysaccharides and polyphenols from Agaricus blazei)

[0036] (1) Raw material pretreatment: After the dried Agaricus blazei fruiting body is ultrafinely pulverized, it is mixed with distilled water in a ratio of 1:30 by weight to form a mixed solution, and then set aside;

[0037] (2) Enzymolysis treatment: add the compound enzyme (cellulase: pectinase: neutral protease=3: 1: 4) that accounts for 0.1% of the mixed solution to the mixed solution, adjust the pH of the mixed solution to be 4, and Put it on a shaker at 45°C for 15 minutes;

[0038] (3) Water extraction treatment: Ultrasonic (200Hz, 800W) treatment of the enzymolysis solution in step (2) for 15 minutes, then heating the solution to 90°C, extracting for 30 minutes, and centrifuging at 6000 rpm for 10 minutes , to obtain supernatant I and precipitate;

[0039] (4) Ethanol extraction: step (3) precipitate is mixed with the ethanol that concen...

Embodiment 2

[0043] Embodiment 2: (method 2 for simultaneously efficiently extracting polysaccharides and polyphenols from Agaricus blazei)

[0044] In this example, step (1) mixes Agaricus blazei ultrafinely pulverized fruiting bodies with distilled water at 1:40 by weight; Protease = 4:1:3) was added to the mixture, at 48°C, pH value 4.5, shaken and enzymatically hydrolyzed for 30 minutes; in step (3), the enzymolyzed solution in step (2) was subjected to ultrasonic (400Hz, 200W) treatment for 20 minutes Minutes later, heat the solution to 95°C and extract for 40 minutes; step (4) mix the precipitate in step (3) with ethanol with a concentration of 55% in a ratio of 1 kg by weight and volume: 40 liters, and shake and extract at 85°C 1 hour; extract and filter the filter residue and 95% ethanol according to the above method; the macroporous resin used in step (6) is PN400. All the other steps, technology are with embodiment 1. Under the above conditions, the polysaccharide yield of Agar...

Embodiment 3

[0045] Embodiment 3: (from Agaricus blazei simultaneously and efficiently extract the method 3 of polysaccharide and polyphenol)

[0046]In this example, step (1) mixes Agaricus blazei ultrafinely pulverized fruiting bodies with distilled water at 1:45 by weight; step (2) will account for 0.3% compound enzyme (cellulase: pectinase: neutral Protease = 5:1:2) was added to the mixture, at 50°C, pH value 5, shaking and enzymolysis for 60 minutes; step (3) microwave (50Hz, 800W) treatment of the enzymolysis solution in step (2) for 25 minutes Minutes later, heat the solution to 95°C and extract for 50 minutes; step (4) mix the precipitate in step (3) with ethanol with a concentration of 55% in a ratio of 1 kg by weight and volume: 45 liters, and shake and extract at 85°C 1 hour; extract and filter the filter residue and 95% ethanol according to the above method; the macroporous resin used in step (6) is CDA40. All the other steps, technology are with embodiment 1. Under the above...

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Abstract

The invention discloses a method for efficiently extracting polysaccharide and polyphenol from agaricus blazei, belonging to the technical field of the method for extracting active ingredients of edible mushroom. The method comprises the following steps: (1) pretreatment of raw material; (2) enzymolysis; (3) water extraction; (4) ethanol extraction; (5) alcohol precipitation of polysaccharide; (6) purification of polyphenol by resin and other steps. In the invention, techniques such as ultramicro pulverization, enzymolysis, auxiliary extraction and resin adsorption are organically combined and polysaccharide and polyphenol with different bioactivities are extracted; in addition, the method of the invention has the characteristics of high yield, high purity, low cost, safety and reliability, thus being of great significance for exploiting potential values of agaricus blazei. In conclusion, the method of the invention can be popularized in health food enterprises.

Description

technical field [0001] The invention relates to the technical field of extraction methods for effective components of edible fungi, in particular to a method for simultaneously and efficiently extracting polysaccharides and polyphenols from edible and medicinal fungus Agaricus blazei. Background technique [0002] Agaricus blazei, also known as Agaricus blazei, is a precious edible fungus, belonging to Basidiomycotina, Phylomycetes, Scadiomycetes, Mushroomaceae, Mushroom genus, and its scientific name is Agaricus blazei Murill. The fungus originated in Brazil, the United States and other places. It was successfully introduced and cultivated in Japan in the 1970s, and was introduced into my country in the early 1990s. Now Fujian, Zhejiang and other places have a large number of cultivation. [0003] At present, my country's Agaricus blazei products are mainly consumed in salted products, dried fruiting bodies and fresh products, and the research and development and applicati...

Claims

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Application Information

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IPC IPC(8): C12P19/04A61K36/07A61P39/06A61P35/00A61P9/00
Inventor 范雷法张作法吕国英潘慧娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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