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Method for enriching and extracting adult stem cells

An adult stem cell and stem cell technology, applied in the biological and medical fields, can solve the problems of high technical requirements, tissue destruction, complicated operation, etc., and achieve the effects of high cell density, simple method and high efficiency

Inactive Publication Date: 2010-07-07
SHANDONG MEDICAL BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method of extracting MDSC is to take out the muscle and cut it into pieces, and then isolate and culture it in vitro. This is a method with serious tissue damage, and at the same time, the operation is complicated, time-consuming, and technically demanding.

Method used

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  • Method for enriching and extracting adult stem cells
  • Method for enriching and extracting adult stem cells
  • Method for enriching and extracting adult stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The gelatin sponge is made into a suitable specification, sterilized by ethylene oxide or other methods, and then implanted in the intermuscular space of the forelimb or hindlimb of a human or animal; after a certain period of time (3 to 15 days), it is taken out under sterile conditions Materials, isolation and culture of cells.

[0026] Purify and isolate the captured cells, use flow cytometry, immunofluorescence, immunoenzyme chemistry and other methods to identify the cell composition, and then perform autologous cell transplantation or use as seed cells for tissue engineering.

Embodiment 2

[0028] The polylactic acid porous three-dimensional tissue engineering material is made into a suitable specification, and after being sterilized by ethylene oxide or other methods, it is implanted in the muscle-muscular space of the forelimb or hindlimb of a human or animal; after a certain period of time (3 days to 15 days), The material is removed under aseptic conditions and the cells are isolated.

[0029] Purify and isolate the captured cells, use flow cytometry, immunofluorescence, immunoenzyme chemistry and other methods to identify the cell composition, and then perform autologous cell transplantation or use as seed cells for tissue engineering.

Embodiment 3

[0031] Make the qualified natural sponge porous three-dimensional tissue engineering into the appropriate specifications for the experiment, sterilize it with ethylene oxide or other methods, and embed it in the human or animal forelimb or hindlimb muscle muscle space; for a certain period of time ( After 3 days to 15 days), the material is removed under aseptic conditions and the cells are isolated.

[0032] Purify and isolate the captured cells, use flow cytometry, immunofluorescence, immunoenzyme chemistry and other methods to identify the cell composition, and then perform autologous cell transplantation or use as seed cells for tissue engineering.

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Abstract

The invention provides a method for enriching and extracting adult stem cells from inside of human being or animals, namely, a physiologically acceptable multihole tri-dimensional tissue engineering material is implanted inside human being or animals to enable the adult stem cells to be enriched on the material, and then the adult stem cells are separated from the materials, purified and proliferated to realize autotransplantation.

Description

technical field [0001] The invention relates to a method for obtaining adult stem cells, in particular to a method for capturing, extracting and enriching muscle stem cells and fat stem cells, which belongs to the technical field of biology and medicine. Background technique [0002] Stem cells are a kind of undifferentiated cells, whose basic characteristics are: self-replicating ability to produce daughter cells identical to itself; differentiation into more than one type of functional cells. According to different stages of differentiation, stem cells can be divided into embryonic stem cells (embryonic stem cells, ESCs) derived from fertilized eggs and other early embryos, and adult stem cells (adult stem cells, ASCs) derived from adult or immature animal tissues. Stem cell research not only has important scientific value for human developmental biology research, but more importantly, it can be used as seed cells for clinical cell therapy and artificial tissue or organ co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08
Inventor 王世立韩金祥丁宁李俊玲
Owner SHANDONG MEDICAL BIO TECH RES CENT
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