Environment-friendly extraction process for extracting dioscin by adopting Rhizopus oryzae

Abstract

The invention relates to an environment-friendly extraction process for extracting dioscin by adopting Rhizopus oryzae, and belongs to the field of biotechnology. The process comprises the following steps: weighing quantitative Dioscorea nipponica powder, adding distilled water of which the volume is twice that of the Dioscorea nipponica powder, and evenly stirring; sterilizing the mixture at a temperature of between 120 and 130 DEG C for 20 to 35min, inoculating a strain of the Rhizopus oryzae, culturing at room temperature for 28 to 35 days, sterilizing, adjusting a pH value to 7 through NaOH, and drying in an oven at a temperature of between 78 and 85DEG C to constant weight; accurately weighing dry powder, putting into a Soxhlet extractor, extracting the dry powder with petroleum ether of which the volume is 8 to 15 times that of the dry powder in a water bath at a temperature of between 78 and 83DEG C, recovering the petroleum ether, and obtaining a crude product of the dioscin. The process has the advantages that: the yield of the dioscin is high; the process is easy to control; the produced dioscin has stable quality; and the process has the most remarkable advantage of saving a step of acid hydrolysis, so the process is environment-friendly and pollution-free in the production process.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] For a long time, people generally use direct acid hydrolysis to extract and separate saponin. This process has the following disadvantages: 1) because the yam rhizome is hard in texture and contains a large amount of starch, it is not easy to hydrolyze thoroughly, and the yield of saponin is low; 2) a large amount of fiber runs through the whole process of production, which increases the consumption cost; Hydrolysis under high temperature and other conditions will destroy other components in yam, which is not conducive to comprehensive utilization. In addition, in the process of acid hydrolysis, it is worth noting that side reactions often occur. First, if hydrochloric acid is used for hydrolysis, hydroxychlorination may occur, so some people suggest that it is best to use sulfuric acid. The second is that diosgenin is easily dehydrated into Δ3,5-deoxytigogenin, and th...

AI Technical Summary

Problems solved by technology

This process has the following disadvantages: 1) because the yam rhizome is hard in texture and contains a large amount of starch, it is not easy to hydrolyze thoroughly, and the yield of saponin is low; 2) a large amount of fiber runs through the whole process of production, which increases the consumption cost; Hydrolysis under high temperature and other conditions will destroy other components in yam, which is not conducive to comprehensive utilization

However, there are many factors affected by this method (enzyme amount, time, temperature, pH value, etc.)

And although this method improves the yield, it can't avoid the acid hydrolysis step in the end, and it will still cause structural damage to other components in the raw material, and the discharge of waste acid water will cause environmental pollution