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Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof

A Bacillus cereus, aerobic denitrification technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as limited proportions

Inactive Publication Date: 2012-04-11
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process control method cannot solve the problem that some strains lack nitrous oxide reductase Nos at all, that is, it cannot fundamentally solve the problem of N 2 The problem of whether O can be reduced, so changes in process operating conditions often improve N 2 The proportion of O produced is very limited, generally improved N 2 O accounts for 10-30% of nitrogen removal from water bodies

Method used

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  • Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof
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  • Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof

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Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Screening of Bacillus cereus CGMCC NO.3047

[0032] This bacterial strain comes from this laboratory, with nitrate as the substrate, the activity in SBR aeration reactor A (total nitrogen TN removal rate 60.0~83.0%, COD removal rate 85.0~90.0%) under high dissolved oxygen condition domestication Sludge, the strain number is WXZ-8, and the preservation number is CGMCC NO.3047.

[0033] Utilizing the characteristics of the bromothymol blue contained in the BTB medium that turns blue when it encounters alkali (as an alkali production indicator), the mixed solution in the laboratory reactor A is evenly coated on the BTB by gradient dilution and coating method Culture medium, after several days of constant temperature and aerobic culture at 30°C, pick a single colony with a blue halo in the surrounding medium and different colony shapes as the primary screened aerobic denitrifying bacteria, and streak on the plate to purify the strain . The pure strain obtained b...

Embodiment 2

[0035] Embodiment 2: Determination method of aerobic denitrification performance of Bacillus cereus CGMCC NO.3047

[0036] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, both the nitrate simulated wastewater I and the nitrate simulated wastewater II were inactivated in a high-temperature sterilization pot of 0.1MP for 20 minutes. Take 50mL of sterilized nitrate simulated waste water I in a 100mL Erlenmeyer flask, put Bacillus cereus (Bacillus cereus) CGMCC NO. bed, the constant temperature is maintained at 30°C, and the shaker rotates at 120-160r / min to activate the strain. After activating for 12-16 hours, inoculate 5% of the inoculum into a 250mL Erlenmeyer flask filled with 200mL nitrate simulated wastewater II to measure the performance of denitrification and denitrification. Every 24 hours, use a needle filter equipped with a 0.2 μm mixed fiber membrane to filter and extract 5 mL of wastewater samples in the Erlenmeyer flask...

Embodiment 3

[0039] Embodiment 3: the determination of aerobic denitrification of Bacillus cereus CGMCC NO.3047

[0040] The conditions for aerobic denitrification of wastewater are: the concentration of dissolved oxygen is achieved by controlling different rotational speeds rpm (r / min). The rotational speed is 100r / min, and the corresponding dissolved oxygen concentration is 4.2-5.0mg / L; the concentration of nitro nitrogen is 126mg / L ; The denitrification performance of the fungus CGMCC NO.3047 was determined under the conditions of carbon-to-nitrogen ratio of 10-25 and pH value of 6.5-7.5.

[0041] Carry out denitrification performance measurement according to embodiment 2 method, test result: NO after 4 days 3 - The reduction rate of -N was 78.0%, and the removal rate of TN (total nitrogen is the sum of nitro-nitrogen and nitro-nitrogen) after 4 days was 76.8%.

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Abstract

The invention discloses a bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and an N2O emission control method thereof, wherein the accession number of the bacillus cereus is CGMCC NO.3047. The bacillus cereus can degrade nitrate under the aerobic condition and perform denitrification; and the bacillus cereus can also grow in the ammonia nitrogen wastewater, perform heterotrophic nitrification-aerobic denitrification by using ammonia nitrogen under the aerobic condition, obtain the gaseous product N2O with extremely low emission amount in the denitrification process and achieve the purpose of N2O emission control from the biological point of view.

Description

technical field [0001] The present invention relates to a bacterial strain with heterotrophic nitrification and aerobic denitrification performance, specifically Bacillus cereus CGMCC NO.3047 with heterotrophic nitrification-aerobic denitrification performance and its N 2 O biological control method. Background technique [0002] Among the various denitrification methods that have been applied so far, biological denitrification is still the main method in wastewater denitrification treatment. Among them, the research on the denitrification effect of heterotrophic nitrifying bacteria is the enrichment and breakthrough of the traditional nitrification-denitrification theory. . Heterotrophic nitrification refers to the process in which heterotrophic microorganisms oxidize ammonia or organic nitrogen compounds to hydroxylamine, nitrite and nitrate under aerobic conditions. The importance of heterotrophic nitrification is attracting increasing attention, especially with good Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/085
Inventor 汪苹尹明锐廖小红项幕飞王磊刘健楠
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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