Preparation method of tilmicosin liposomes

Abstract

The invention relates to a preparation method of tilmicosin liposomes. In the technical scheme, every 3g of tilmicosin raw powder is processed into tilmicosin liposomes by the following steps: (1) preparing a PBS buffer solution with the pH value of 6.5 to 7.5; (2) mixing egg yolk lecithin, cholesterol, octadecylamine, polysorbate-80 and ether in the proportion into a solution; (3) mixing the tilmicosin raw powder with the PBS buffer solution obtained in the step (1), and then pouring the mixture into the solution prepared in the step (2) and uniformly stirring; pulverizing the mixture by ultrasound into a suspension which does not delaminate within at least 5min; (4) transferring the suspension in the step (3) into a rotary evaporator to perform pressure-reduction rotary evaporation so that the suspension is condensed on the wall of the rotary evaporator to form lipid membranes; and (5) washing off the lipid membranes completely with the PBS solution obtained in the step (1), and thencontinuing the pressure-reduction evaporation until the ether is drained entirely to obtain tilmicosin liposome crude products. The method has the advantages of simple steps and strong operability. The prepared tilmicosin liposomes can be easily absorbed after being injected into livestock and poultry organisms; and the phenomenon of local swelling can not be caused.

Description

technical field [0001] The invention belongs to the field of antibiotic preparations, and in particular relates to a preparation method of tilmicosin liposome dosage form. Background technique [0002] Tilmicosin is a semi-synthetic macrolide antibiotic for livestock and poultry developed by the British Elanco company in the 1980s. It has been approved abroad for the treatment of infectious diseases in pigs, cattle, sheep, chickens and other animals. Diseases, especially livestock and poultry respiratory infections, such as livestock actinobacillus pleuropneumonia, pasteurellosis and chicken mycoplasma, etc., are effective to gram-positive bacteria, some gram-negative bacteria, mycoplasma and spirochetes inhibition. Compared with tylosin, tilmicosin has stronger antibacterial activity, less dosage (it is reported that the dosage of subcutaneous or intravenous injection is 10-20 mg / Kg body weight, once effective), long-lasting effect and other advantages. It is an economical...

AI Technical Summary

Problems solved by technology

[0004] In recent years, there have been many reports on liposomes of drugs such as amikacin and florfenicol at home and abroad, but there are very few reports on liposomes of tilmicosin, and the preparation method is relatively simple.