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Nerve growth factor sponginum and preparation method thereof

A nerve growth factor and sponge technology, applied in the field of medicine, can solve the problems of difficulty in achieving sustained release, cumbersome operation process, poor wound adhesion, etc., and achieves the effects of rich raw material sources, simple production process and reduced damage.

Inactive Publication Date: 2009-12-30
WUHAN HITECK BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(Chinese patent application 200310111375.7, disclosed on November 10, 2004) but human blood bioglue has the following disadvantages in clinical application: 1. it needs to be dissolved and prepared in advance under the operating table before use, and a spray device needs to be assembled, and the operation process is loaded down with trivial details, Time-consuming; ②Poor adhesion to the wound surface, easily washed off by blood or tissue fluid; ③Because it is prepared from plasma, it is expensive to manufacture and the clinical price is high
After the preparation is implanted in the body, the drug that is not fully adsorbed on the matrix material or has a weak adsorption will rapidly diffuse from the matrix and cause burst release, so it is difficult for this preparation to achieve a better sustained release effect.

Method used

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  • Nerve growth factor sponginum and preparation method thereof
  • Nerve growth factor sponginum and preparation method thereof
  • Nerve growth factor sponginum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Extraction and preparation of collagen

[0048] (1) Take the tendons of pork legs, remove impurities such as epidermis and fat, and clean them with cold purified water after slicing;

[0049] (2) Soak and wash with 20% sodium chloride for 3 times to degrease and remove impurities such as serum;

[0050] (3) Dissolve in 60 times the volume of 1% acetic acid solution, add pepsin at a final concentration of 0.4%, stir and digest at 37°C for 1 hour, then place it at 2-8°C for 5 days;

[0051] (4) Centrifuge the digestion solution, collect the supernatant, add NaCl to a final concentration of 2mol / L, precipitate overnight at 2-8°C, and collect the precipitate; redissolve the precipitate in 1% acetic acid solution, collect the supernatant by centrifugation, and add NaCl To a final concentration of 2 mol / L, precipitate overnight at 2-8°C, collect the precipitate, and obtain collagen.

Embodiment 2

[0052] Embodiment 2: Extraction and preparation of collagen

[0053] (1) Take the tendons of horse legs, remove impurities such as epidermis and fat, and clean them with cold purified water after slicing;

[0054] (2) Soak and wash with 20% sodium chloride twice to degrease and remove impurities such as serum;

[0055] (3) Dissolve in 30 times the volume of 1% acetic acid solution, add pepsin at a final concentration of 0.4%, stir and digest at 37°C for 1 hour, then place it at 2-8°C for 10 days;

[0056] (4) Centrifuge the digestion solution, collect the supernatant, add NaCl to a final concentration of 2mol / L, precipitate overnight at 2-8°C, and collect the precipitate; redissolve the precipitate in 0.1% acetic acid solution, collect the supernatant by centrifugation, and add NaCl To a final concentration of 1mol / L, precipitate overnight at 2-8°C, collect the precipitate, and obtain collagen.

Embodiment 3

[0057] Embodiment 3: Extraction and preparation of collagen

[0058] (1) Take the calf tendon, remove impurities such as epidermis and fat, and clean it with cold purified water after slicing;

[0059] (2) Soak and wash with 20% sodium chloride for 3 times to degrease and remove impurities such as serum;

[0060] (3) Dissolve in 50 times the volume of 1% acetic acid solution, add pepsin at a final concentration of 0.4%, stir and digest at 37°C for 1 hour, then place it at 2-8°C for 7 days;

[0061] (4) Centrifuge the digestion solution, collect the supernatant, add NaCl to a final concentration of 2mol / L, precipitate overnight at 2-8°C, and collect the precipitate; redissolve the precipitate in 0.5% acetic acid solution, collect the supernatant by centrifugation, and add NaCl To a final concentration of 1.5 mol / L, precipitate overnight at 2-8°C, collect the precipitate, and obtain collagen.

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Abstract

The invention relates to a nerve growth factor sponginum and a preparation method thereof, belonging to the technical field of medicine. The sponginum comprises nerve growth factor with weight percent content being 0.01-2% and collagen sponge or gelatin sponge matrix, the nerve growth factor is fixed on the collagen sponge or gelatin sponge matrix in a crosslinking way by an aldehydes crosslinker. The sponginum can cause the nerve growth factor to be released to the nerve pathology or wound tissue partial position continuously for a long time in effective dose, and play a role in effectively curing nerve tissue damage.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a nerve growth factor sponge and a preparation method thereof. The sponge can release the nerve growth factor to the local position of the neuropathy or wound tissue in an effective dose for a long time, and play an effective role in treating nerve tissue damage. Background technique [0002] Nerve Growth Factor (NGF) is the earliest neurotrophic factor discovered and the most thoroughly studied at present. It is a kind of nerve cell growth regulator with dual biological functions of neuron nutrition and promotion of neurite growth. The development, differentiation, growth, regeneration and expression of functional properties of peripheral neurons all play important regulatory roles. There are many sources of NGF preparation, including snake venom, guinea pig prostate, bovine seminal plasma, human placenta, mouse submandibular gland, etc. The content of male mouse submandibular g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K38/18A61K47/48A61K47/42A61P25/00C07K14/78C07K1/14
Inventor 汤华东陈亚李汝霖
Owner WUHAN HITECK BIOLOGICAL PHARMA
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