Method and kit for detecting polymorphism of ADPRT gene

Abstract

The invention discloses a method for detecting the polymorphism of an ADPRT gene, which is characterized by comprising the following steps: (a) human genomic DNA is used as a template, and a positive primer and a reverse primer of a gene sequence are designed in a region of an ADPRT gene locus; a single basic group is mismatched on the positive primer so as to introduce an enzyme cutting site; and the template genomic DNA is processed by specificity polymerase chain reaction (PCR) amplification; (b) a restriction enzyme is utilized to carry out enzyme cutting on the genomic DNA; and (c) segment size separation is carried out on the ADPRT gene according to agarose gel electrophoresis so as to determine the gene polymorphism of the ADPRT gene. The invention also discloses a kit for detecting the ADPRT gene polymorphism, which comprises a gene polymorphism primer for amplification, dNTPs, DNA polymerase for the PCR reaction, a buffer solution of the DNA polymerase, a restriction enzyme Bsh1236I for restriction fragment length polymorphism (RFLP) and a corresponding buffer solution. The method has simple operation, low cost, intuitional judging result, quick reaction and convenient popularization and application, and the kit is convenient to use.

Description

technical field [0001] The invention relates to a gene detection method, in particular to a detection method for detecting ADPRT gene polymorphism and a kit for the method. Background technique [0002] The occurrence and evolution of tumors is a multi-stage and multi-step process of interaction between environmental factors and genetic factors. From a macroscopic point of view, this process reflects the interaction between the environment and the host, especially the genetic factors of the host; while from the microscopic point of view, the occurrence of tumors essentially reflects the influence and effect of carcinogenic factors on the genetic material of cells. . Studies have shown that among people living in the same environment, some suffer from cancer, while others are safe. Even in some areas with high tumor incidence, the actual incidence of tumor is only about 0.1-0.2%. Over the years, in the study of tumor etiology, a large amount of data has been accumulated, in...

AI Technical Summary

Problems solved by technology

[0006] In recent years, people have developed many methods for detecting gene polymorphism, the more common ones are single-strand conformation polymorphism (SSCP), direct sequencing technology, denaturation-high pressure liquid chromatography (DHPLC), dynamic allele-specific Hybridization technology (DASH) and TagMan technology, etc., many of which have obvious advantages in high-throughput detection, but due to expensive instruments, corresponding technical platform support, and relatively high detection costs, they are not suitable for Mutation detection of known loci in small samples