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Method for building psoriasis basic research models, and gas-liquid level transmembrane device

A technology for basic research and establishment of methods, which is applied in the field of experimental model establishment to achieve the effect of saving expenses and making preparations simple and easy.

Inactive Publication Date: 2009-11-04
刘晓明 +1
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Problems solved by technology

Maintains characteristic tissue morphology of psoriatic lesions for up to a week

Method used

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  • Method for building psoriasis basic research models, and gas-liquid level transmembrane device
  • Method for building psoriasis basic research models, and gas-liquid level transmembrane device
  • Method for building psoriasis basic research models, and gas-liquid level transmembrane device

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Embodiment Construction

[0047] 1. Materials collection: ①Skin sample collection: take untreated psoriasis vulgaris skin lesions within half a year; ②Psoriasis peripheral blood collection: take 2mL peripheral blood from corresponding psoriasis patients, collect in sterile vacuum anticoagulant blood collection tubes, The collection of blood samples and all skin samples obtained the informed consent of the patients and was approved by the ethics committee; ③β-hemolytic streptococci

[0048] 2. Material handling and transportation: take the skin lesion under aseptic operation, the size is about 1.5cm×1.5cm, remove the sebum and part of the dermis, put it into a 1.5mL centrifuge tube containing 200ul of William's medium containing 10% penicillin and streptomycin, Ship to the laboratory within 30 minutes at 4°C; ship blood samples to the laboratory within 30 minutes at 37°C.

[0049] 3. Separation of T lymphocytes: Take 2 mL of fresh anticoagulated blood, mix it with PBS 1:1, carefully add it to 4 mL of ly...

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Abstract

The invention provides a method for building psoriasis basic research models, and a gas-liquid level transmembrane device. The method utilizes a psoriasis streptococcal-induced mechanism and a T cell-mediated excessive proliferation mechanism, innovatively adopts a serum-free medium added with psoriasis peripheral blood T lymphocytes and hemolytic streptococcus antigens, and cultures psoriasis skin lesions in vitro. Characteristic tissue morphology of the psoriasis skin lesions can be maintained for as long as one week. The gas-liquid level transmembrane device used for culturing psoriasis in vitro skin lesions comprises the serum-free medium containing psoriasis peripheral blood T cells and the hemolytic streptococcus antigens in the method for building psoriasis basic research models, as well as sterilizing lens-wiping paper for manufacturing gas-liquid level, wherein tissue blocks and a gas phase are borne on the sterilizing lens-wiping paper; the gas phase is at 37 DEG C with 5 percent of CO2 saturation. The device not only can completely simulate gas-liquid junction environment for human skin growth, but also can reach the requirements of in vitro skin tissue culture. In addition, the device is easy to prepare, saves expenses, and has the advantages of repeatability, controllability and the like.

Description

technical field [0001] The present invention belongs to the field of establishment of experimental models. Background technique [0002] With the in-depth and extensive research on psoriasis, the in vitro model of psoriasis as the basis of the experiment has always been a hot spot in the academic circle. On the one hand, people are committed to the research of psoriasis mouse model: as early as the 1960s, people have begun to use ultraviolet rays and chemical agents to induce the establishment of mouse psoriasis models. However, this model only simulates a specific pathology of psoriasis, and it has short-lived defects. [0003] In the 1970s, Krueger and others successfully transplanted human psoriasis lesions into nude mice for the first time, but Baker and others evaluated the transplanted skin and found that most of the characteristics of psoriasis could not be maintained for a long time after transplantation. factors that affect the experimental results. It is maintai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12M3/02C12Q1/02
Inventor 刘晓明王曼姬
Owner 刘晓明
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