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I-type medical collagen material keeping original specific triple helix structure of collagen, product and application thereof

A collagen material and triple helix technology, which is applied to the type I medical collagen material that maintains the unique triple helix structure of collagen and its products and application fields, can solve the problems of scar tissue formation, affecting the treatment effect, and the treatment failing to achieve the expected effect. , to achieve the effect of reducing tissue adhesion, low immune antigenicity, and no foreign body reaction

Active Publication Date: 2012-12-05
许和平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty in obtaining the own tissue, or the insufficient selection of materials, the treatment often fails to achieve the expected effect, while other substitutes often have a high immune response, and will form scar tissue, affecting the treatment effect

Method used

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  • I-type medical collagen material keeping original specific triple helix structure of collagen, product and application thereof
  • I-type medical collagen material keeping original specific triple helix structure of collagen, product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Extraction of type I medical collagen materials that maintain the unique triple helix structure of collagen

[0025] Choose 1kg of bovine Achilles tendon tissue, freeze it, cut it into thin slices with a thickness of 0.1-3 mm, and immerse it in 0.1% sodium dihydrogen phosphate / sodium hydroxide solution for 1-3 hours under stirring conditions. Keep its pH neutral. Add 1:5 (w / w) ficin enzyme to the above-mentioned treated substance solution to fully react. With periodic stirring, thereafter treated with 1% ammonium nitrate for 6 hours. Then wash. Then add 1M sodium chloride for further treatment and control the temperature, adjust the pH, let stand overnight at 4°C, and then react with 2M equivalent sodium hydroxide solution. Then use 50% sulfuric acid to neutralize the buffer and adjust the pH to make it slightly acidic, take out the precipitate, wash and soak for 6 hours, then remove the water, and obtain the type I medical collagen material that maintains...

Embodiment 2

[0027] Embodiment 2: Preparation of meninges / spinal membranes biofilm

[0028] Take an appropriate amount of the type I medical collagen material with a triple helix structure in Example 1, dissolve 3.6 grams of collagen in 720 milliliters of 0.1M glacial acetic acid to make a 0.5% concentration of collagen suspension, and stir under high-speed and low-temperature conditions (20,000 rpm; 4°C) Add 6-chondroitin sulfate within 2 hours to obtain a 0.5% composite suspension, pour 720 ml into a 30×150 cm freezer tray, and put it in a freeze dryer freezer Freeze dried.

[0029] The setting of the freezing temperature is (1) -7°C / 30 minutes / in a continuous state; (2) -40°C / 200 minutes / in a curve state; (3) -40°C / 70 minutes / in a continuous state; freezing during the process The temperature of the dryer freezer was maintained at -80°C. The temperature of the sublimation stage was set to (1) 0°C / 18 hours; (2) the temperature of the second heating was set to 20°C for 40 minutes, and th...

Embodiment 3

[0032] Embodiment 3: Preparation of meninges / spinal membrane biofilm

[0033] Take an appropriate amount of the type I medical collagen material with a triple helix structure of Example 1, dissolve 7.2 grams in 0.1M glacial acetic acid of 720 milliliters, and make a collagen suspension with a concentration of 2%, under high-speed low-temperature stirring conditions ( 30,000 rpm; 0°C) Add 6-chondroitin sulfate within 1.5 hours to obtain a 1% compound suspension, take 125 ml and pour it into a 12.5×12.5 cm freezer tray, and put it in a freeze dryer freezer for freezing dry.

[0034] The setting of the freezing temperature is (1) -9°C / 30 minutes / in a continuous state; (2) -50°C / 220 minutes / in a curve state; (3) -45°C / 80 minutes / in a continuous state; freezing during the process The temperature of the dryer freezer was maintained at -80°C. The temperature in the sublimation stage was set to (1) 0°C / keep for 20 hours; (2) the temperature for the second heating was set to 20°C for...

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Abstract

The invention relates to an I-type medical collagen material keeping an original specific triple-helical structure of collagen, and an extraction method thereof, meninges / spinal meninges biomembrane made from the I-type medical collagen material, and a preparation process of the biomembrane, and application of the biomembrane to preparing meninges / spinal meninges tissue repair materials. The I-type medical collagen material keeping the original specific triple helix structure of collagen has the advantages of low immunogenicity, no foreign body reaction, good biocompatibility and controllabledegradation rate, the prepared meninges / spinal meninges biomembrane has certain stretching resistance strength, repairable and regenerative dura mater / spinal meninges tissue and tissue adhesion prevention / reduction, and the biomembrane is applicable to repair and regeneration of injured cerebral dura mater and spinal dura mater.

Description

technical field [0001] The present invention relates to the technical fields of medicine, medical biomaterials, medical tissue engineering and regenerative medicine, in particular to a type I medical collagen material which maintains the unique triple helix structure of collagen and an extraction method thereof, and the type I medical collagen material is used to prepare A meninges / spinal membrane biofilm, a preparation process of the biofilm and its application in preparing materials for repairing meninges / spinal tissue and preventing or reducing tissue adhesion. Background technique [0002] At the end of the 20th century, with the rapid development of holistic medicine, cell biology, molecular biology, genetics, tissue engineering, and regenerative medicine also continued to progress and develop. Biomaterials with good properties are also being discovered and utilized rapidly. This is proved by the rapid rise of tissue engineering and regenerative medicine in clinical me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/26A61L27/54A61L27/56A61L27/58A61L27/20
Inventor 许和平
Owner 许和平
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