Method for preparing ready-to-use tea fungus starter
A kombucha and starter technology, applied in the direction of microbial-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problem of inconvenient recovery and cleaning of bacterial film, affecting normal fermentation, and difficult control And other issues
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specific Embodiment approach
[0026] The specific embodiment: the specific embodiment of the present invention is described in detail below:
[0027] 1. Strains:
[0028] Acetic acid bacteria: Acetobacter aceti subsp. xylinum; other similar acetic acid bacteria such as Acetobacter gluconeobacterium, Acetobacter ketogenicum, Acetobacter suboxidans, and Acetobacter pasteurii can also be used.
[0029] Lactic acid bacteria: Lactobacillus bulgaricus, Bifidobacterium; Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and other similar lactic acid bacteria can also be used.
[0030] Yeasts: Saccharomyces pasturii, Saccharomyces cerevisiae; other similar yeasts can also be used.
[0031] 2. Preparation of culture medium:
[0032] Yeast broth:
[0033] The existing potato starch medium and malt extract medium can be used, and the potato starch medium prepared by the following method can also be used: wash and peel the potatoes, cut them into small pieces, weigh 200g, add 200g of water ...
Embodiment 1
[0039] Insert Saccharomyces pasteur and Saccharomyces cerevisiae into the above-mentioned yeast liquid culture medium, culture them statically at 22°C for 2 days, harvest the cells by centrifugation and wash them twice with sterile water;
[0040] Acetobacter aceti subsp. xylinum was inserted into the above-mentioned liquid culture medium of acetic acid bacteria, and the rotation speed was 220rpm on a rotary shaker; the temperature was 30°C, cultured for 2 days, and the cells were harvested by centrifugation and washed twice with sterile water;
[0041] Insert Lactobacillus bulgaricus and Bifidobacterium into the above-mentioned lactic acid bacteria liquid medium, rotate the shaker, rotate at 220rpm; temperature 37°C, cultivate for 2 days, harvest the bacteria by centrifugation and wash twice with sterile water;
[0042] Preparation of kombucha culture solution: Take 150g of sucrose, 20g of Pu’er tea, and 830g of water, boil the water, and when the boiling water is cooled to 85...
Embodiment 2
[0051] Insert Yeast Pasteur into the above yeast liquid medium, culture it statically at 20°C for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;
[0052] Insert Acetobacter glucosus and Acetobacter ketogenes into the above-mentioned acetic acid bacteria liquid culture medium, rotate on a shaker, rotate at 220rpm; temperature 25°C, culture for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;
[0053] Insert Streptococcus thermophilus and acidophilic lactic acid bacteria into the above-mentioned lactic acid bacteria liquid medium, rotate the shaker, rotate at 220rpm; temperature 35°C, cultivate for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;
[0054] Preparation of kombucha culture solution: Take 50g of sucrose, 2g of Longjing tea, and 948g of water, boil the water, and when the boiling water is cooled to 85°C-90°C, add tea, keep it warm for 20min, filter out the tea dregs, and the...
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