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Method for preparing ready-to-use tea fungus starter

A kombucha and starter technology, applied in the direction of microbial-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problem of inconvenient recovery and cleaning of bacterial film, affecting normal fermentation, and difficult control And other issues

Inactive Publication Date: 2009-10-21
张睿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. The research on the strains of kombucha and the dominant groups of the strain combination has not yet started:
[0008] In the production of traditional kombucha beverages, because the fermented kombucha has a membranous structure and has the ability to reproduce, and the relationship between bacteria and bacteria is not constant, it is in a state of change, and it is easily affected by the external environment and easily polluted , affecting normal fermentation, it has the disadvantages of being variable, difficult to control, inconvenient to recover and clean the bacterial film, unstable fermentation performance, etc.

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach

[0026] The specific embodiment: the specific embodiment of the present invention is described in detail below:

[0027] 1. Strains:

[0028] Acetic acid bacteria: Acetobacter aceti subsp. xylinum; other similar acetic acid bacteria such as Acetobacter gluconeobacterium, Acetobacter ketogenicum, Acetobacter suboxidans, and Acetobacter pasteurii can also be used.

[0029] Lactic acid bacteria: Lactobacillus bulgaricus, Bifidobacterium; Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and other similar lactic acid bacteria can also be used.

[0030] Yeasts: Saccharomyces pasturii, Saccharomyces cerevisiae; other similar yeasts can also be used.

[0031] 2. Preparation of culture medium:

[0032] Yeast broth:

[0033] The existing potato starch medium and malt extract medium can be used, and the potato starch medium prepared by the following method can also be used: wash and peel the potatoes, cut them into small pieces, weigh 200g, add 200g of water ...

Embodiment 1

[0039] Insert Saccharomyces pasteur and Saccharomyces cerevisiae into the above-mentioned yeast liquid culture medium, culture them statically at 22°C for 2 days, harvest the cells by centrifugation and wash them twice with sterile water;

[0040] Acetobacter aceti subsp. xylinum was inserted into the above-mentioned liquid culture medium of acetic acid bacteria, and the rotation speed was 220rpm on a rotary shaker; the temperature was 30°C, cultured for 2 days, and the cells were harvested by centrifugation and washed twice with sterile water;

[0041] Insert Lactobacillus bulgaricus and Bifidobacterium into the above-mentioned lactic acid bacteria liquid medium, rotate the shaker, rotate at 220rpm; temperature 37°C, cultivate for 2 days, harvest the bacteria by centrifugation and wash twice with sterile water;

[0042] Preparation of kombucha culture solution: Take 150g of sucrose, 20g of Pu’er tea, and 830g of water, boil the water, and when the boiling water is cooled to 85...

Embodiment 2

[0051] Insert Yeast Pasteur into the above yeast liquid medium, culture it statically at 20°C for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;

[0052] Insert Acetobacter glucosus and Acetobacter ketogenes into the above-mentioned acetic acid bacteria liquid culture medium, rotate on a shaker, rotate at 220rpm; temperature 25°C, culture for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;

[0053] Insert Streptococcus thermophilus and acidophilic lactic acid bacteria into the above-mentioned lactic acid bacteria liquid medium, rotate the shaker, rotate at 220rpm; temperature 35°C, cultivate for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;

[0054] Preparation of kombucha culture solution: Take 50g of sucrose, 2g of Longjing tea, and 948g of water, boil the water, and when the boiling water is cooled to 85°C-90°C, add tea, keep it warm for 20min, filter out the tea dregs, and the...

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PUM

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Abstract

The invention discloses a method for preparing a ready-to-use tea fungus starter, which is characterized by comprising the following steps: mixing yeast thalli, acetic bacteria thalli and lactic-acid bacteria thalli, which are obtained by centrifuging in equal proportion, inoculating the thalli into tea fungus culture fluid to be cultured and then mixing with sodium alginate solution in equal volume, dropping the solution into CaCl2 solution, placing obtained bacteria-containing gel beads into the CaCl2 solution and standing to prepare the ready-to-use tea fungus starter. The method has the advantages of short production cycle, high yield, no waste and the like. The ready-to-use tea fungus starter prepared by the method solves the problems of variable microflora and complex process of traditional tea fungi in the industrialized production and provides a reliable approach for the industrialized production of tea fungus beverage. As the thalli used by the ready-to-use tea fungus starter are exact probiotics which are non-toxic to humans, functional drinks produced by the ready-to-use tea fungus starter, which are secure in health-care property and security can be used.

Description

Technical field: [0001] The invention relates to a preparation method of a direct-throwing type kombucha starter, which belongs to the field of bioengineering or food engineering. Background technique: [0002] Kombucha is also known as "Haibao" and "Weibao". Brewing kombucha with black tea water can produce beverages containing lactic acid, tea polyphenols, vitamins and other natural nutrients. Studies have found that kombucha can improve gastrointestinal function, Anticancer, antibacterial, immune and other aspects have good performance. But it has not been able to form a large-scale industrial promotion so far. The specific reasons are as follows: [0003] 1. The research on the health care performance of kombucha is not thorough enough: [0004] There are few research reports on the health function and safety of kombucha in China. Only Chen Xuan and others reported the effect of active ingredients in tea and kombucha on the ecological balance of human intestinal tract...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12R1/225C12R1/865C12R1/645C12R1/02C12R1/46C12R1/23C12R1/245C12R1/01
Inventor 张睿
Owner 张睿
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