System for quantitative detection of quanta dot mark test bar and detection method thereof

Abstract

The invention provides a system for the quantitative detection of a quanta dot mark test bar and a detection method thereof. The system comprises a quanta dot mark test bar, a test bar rack, a scanning system, a photovoltaic conversion system, an analog-to-digital converter, a data processing device, an output display device, a printer, a keyboard and an IC card matched with the test bar. The data processing device reads the parameters of the IC card and then controls the test bar rack to move so as to make the scanning system automatically scan a test bar detection band and a quality controlband, the acquired signals are transmitted back to the data processing device by the photovoltaic conversion system and the analog-to-digital converter, so that the optical density values of the detection band and the quality control band undergo recognition, concentration counting and technical analysis, and the detection results are finally transmitted to the output display device for display. The system has the advantages of high detection sensitivity, objective results, flexible use, and the like.

Description

technical field [0001] The invention relates to a quantum dot labeled test strip quantitative detection system and a detection method thereof. The system can scan, detect and interpret quantum dot-labeled test strips to realize biological detection of pathogens, antigens, antibodies, prohibited drugs, major diseases (tumor, cancer, cardiovascular disease, diabetes, etc.), pesticide residues, and food safety. Quantitative, semi-quantitative and qualitative detection of various target objects. Background technique [0002] In immunoassay, methods such as radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeling are currently mainly used to achieve quantitative or qualitative detection of samples. These methods have various defects. [0003] Quantum Dot (Quantum Dot, referred to as QD) is a semiconductor nanocrystal developed in the 1990s with excellent spectral characteristics and photochemical stability. It is similar to biomolecules and has t...

AI Technical Summary

Problems solved by technology

The disadvantages are: (1) The patent application number only describes the production of quantum dot marking test strips, but does not disclose the corresponding matching detection system. The detection can only be carried out with the help of other ultraviolet detectors, and it is only a qualitative detection. Quantitative detection cannot be achieved

(2) The use of ultraviolet detection is harmful to the human body and unsafe to operate

(3) The objectivity of the measurement results is poor

Since the tester judges the test result by observing the fluorescence intensity of the test strip and the quality control zone under ultraviolet light with the naked eye, the test result is easily affected by the tester's experience and is highly subjective. The same test strip is in different states. It is possible to make different or even wrong judgment results, especially for those samples with extremely low target content, the fluorescence emitted by ultraviolet light is very weak, it is difficult to judge the results with the naked eye, and the sensitivity is low

(4) Cannot monitor the dynamic process of biological reactions

Immunochromatography is a dynamic reaction process. The fluorescence produced by the quantum dot label particles in the siphon migration state on the test strip belongs to the dynamic category. Obviously, the ultraviolet visual method cannot observe and analyze this dynamic reaction process, so it is impossible to go deeper. To study the immunochromatographic reaction process

Even if the biological reaction fails, the detector cannot analyze the reason for the failure of the reaction well

[0011] Chinese patent application No. 200610024566.3 discloses a method of combining quantum dot fluorescent probes to detect biochips to find Chinese medicine targets. This patent also has shortcomings: the detection of the "quantum dot-Chinese medicine ingredients" fluorescent probes involved not only requires other technologies to provide Corresponding protein chips, and its detection also requires additional special equipment

[0012] Chinese patent application number 200510059892.3 discloses an optoelectronic rapid diagnostic test system including quantum dot test strip detection, but this patent has not yet developed a ready-made product, and the content is completely different from the present invention

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