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Method for extracting pine nut protein of Korean pine

A protein extraction and pine nut technology, applied in the field of Korean pine pine nut protein preparation, can solve the problems of low activity and low extraction rate of Korean pine pine nut protein, etc., and achieve the effects of high activity, increased additional economic value, and high extraction rate.

Inactive Publication Date: 2009-08-26
王振宇
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of low extraction rate and low activity of Korean pine nut protein, the invention provides a method for preparing Korean pine nut protein by a strong electric field method, which can effectively improve the extraction rate and product activity of Korean pine nut protein

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: in this specific embodiment, one, the preparation of Korean pine pine nuts: first carry out the screening of Korean pine pine nuts, choose fresh Korean pine pine nuts, then shell, remove red coat, then dry with tunnel drier . Two, degreasing: pulverize the Korean pine pine nuts, add the Korean pine pine nuts powder and 90% ethanol respectively with the ratio of 1g: 15mL~1g: 20mL with the material-liquid ratio, stir and soak for 24h, remove the soaking liquid, and soak the The pine nut solid powder is put into supercritical extraction equipment for degreasing again. 3. Extraction of Korean pine pine nut protein: add the degreased Korean pine pine nuts in a ratio of 1g: 15mL ~ 1g: 20mL to pure water, carry out strong electric field extraction for 60min, and finally use a decanter centrifuge at 1800 ~ 2500r / min The solid-liquid separation is carried out at a high speed, and the extract and residue are separated, and the residue is added with 60% e...

specific Embodiment approach 2

[0007] Specific embodiment two: this specific embodiment differs from specific embodiment one in that it is characterized in that the preparation of the Korean pine pine nuts in step one, the tunnel drying temperature is 45°C, the drying time is 30-40min, and the water content of the pine nuts is 3%- 5%. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Embodiment 3: This embodiment is different from Embodiment 1 in that it is characterized in that the supercritical degreasing temperature in step 2 is 30-35°C, the pressure is 20-30Mpa, and the time is 2-3h. Others are the same as Embodiment 1. .

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Abstract

The invention provides a method for extracting pine nut protein of the Korean pine, relating to a preparation method of pine nut protein of the Korean pine. The invention solves the problems of the prior traditional method that extraction yield of the pine nut protein of the Korean pine is low, activity of the extracted pine nut protein of the Korean pine is not high and the processing technique is complicated. The method has the following technologic steps: selecting pine nuts of the Korean pine, decorticating pine nuts, removing red shell of the pine nuts, grinding, degreasing, soaking and extracting the pine nutsin a highfield; using an overspeed centrifugal machine to separate the extracting solution followed by concentrating; freezing out the extracting solution by a vacuum freeze dryer, thus obtaining the freeze-dried powder of the pine nut protein of the Korean pine. The pine nut protein of the Korean pine prepared in the invention is a natural extractive which has no side effect on human body and has the effects of resisting aging, lengthening longevity, losing weight, lowering blood fat level, resisting cancer, nourishing the five internal organs, resisting fatigue, beautifying and the like. The pine nut protein of the Korean pine prepared in the invention is applicable to food, health products, cosmetics, medicine, biochemistry and other fields.

Description

technical field [0001] The invention relates to a preparation method of Korean pine nut protein. Background technique [0002] Korean pine pine nut protein is a special active factor of Korean pine pine nuts, mainly composed of albumin. [0003] Because the Korean pine pine nut protein has important physiological functions for the human body, it has an anti-oxidation effect, and it can greatly improve people's physical strength, energy, endurance, appearance and life as a whole. It can enhance the body's immunity, promote the growth and development of the body, and has the functions of anti-aging, prolonging life, losing weight, lowering blood fat, anti-cancer, moisturizing the five internal organs, anti-fatigue, and beautifying. However, the content of this protein in Korean pine pine nuts only accounts for about 10%, and the purified products of Korean pine pine nut protein are relatively rare at present. As the main active ingredient in many products of Korean pine pine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415
CPCY02P20/54
Inventor 王振宇焦岩
Owner 王振宇
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