Antimicrobial peptide gene of portunus trituberculatus and clone method

Abstract

The invention provides a Portunus trituperculatus antibacterial peptide gene and a cloning method thereof. The Portunus trituperculatus crustin antibacterial peptide gene cDNA is 565nt except poly A, and contains 5' non-coding region (5' UTR) of 75nt, a reading frame of 330nt, and 3' non-coding region (3' UTR) of 160nt, wherein the sequence number of GenBank is FJ467931. The method successfully clones complete sequence of Portunus trituperculatus crustin cDNA, can construct pronucleus or eucaryon gene engineering strain through a gene engineering mode, efficiently express recombinant protein with physiological activity, and can be produced in scale. As a feed immunity additive, the gene can replace certain antibiotics in the traditional feed for seaculture industry, thereby realizing green culture, reducing and even eliminating accumulation of the antibiotics in aquatic products, and improving quality of the aquatic products; the gene can also be used as an effective substitute of certain drug resistance antibiotics for the medicine of preventing and treating drug resistance bacteria in seaculture.

Description

technical field [0001] The invention relates to a type I Crustin antimicrobial peptide gene cloned from Portunus trituberculatus and belongs to the field of biotechnology. Background technique [0002] China is the largest aquaculture country in the world. The total output of aquatic products (fishing + aquaculture) accounts for 35% of the world, of which the output of aquaculture accounts for about 70% of the world. In 2005, the output of aquatic products reached 51.81 million tons. Among them, 28.54 million tons of marine products, accounting for 55% of the total output, about 10.22 million tons of fishing, about 18.88 million tons of artificial breeding products. With the global consumption of aquatic products increasing year by year, domestic consumption is growing rapidly. On the other hand, global marine fishing resources are declining, and the supply is gradually shifting to farmed aquatic products. China's aquaculture industry is becoming more and more important in g...

AI Technical Summary

Problems solved by technology

However, with the development of the portunus aquaculture industry, the diseases of the portunus crab, like other cultured species, show an increasing trend year by year, such as vibriosis, gill rot, crustaceous ulcer disease, milk disease, etc., causing heavy losses to the portunus aquaculture industry and becoming a constraint An important factor for the healthy development of the industry

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