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Use of rare earth fluoride nano material in MALDI-MS

A technology of rare earth fluorides and nanomaterials, which is applied in the fields of analyzing materials, analyzing materials through electromagnetic means, measuring devices, etc., and achieves the effects of simple preparation methods, high yields, and strong versatility

Inactive Publication Date: 2009-08-12
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the use of rare earth fluoride nanomaterials as the matrix of MALDI is still in its infancy, and there is no report on the use of rare earth fluoride as the matrix of MALDI at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Rare earth fluoride nanomaterials used as MALDI-MS matrix for mass spectrometric analysis of organic acids

[0039] Preparation of Rare Earth Fluoride Nanomaterials

[0040] method 1.

[0041] Weigh 1mmol rare earth metal oxide (Ln 2 o 3, Ln=Y, La, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu) added into 5ml of nitric acid solution with a concentration of 1mol / L; Add amine tetraacetic acid to 5.3-7.8ml of ammonia solution with a concentration of 1mol / L. After it is completely dissolved, mix the two solutions, add 1-2ml of water and 10ml of absolute ethanol, and sonicate in an ultrasonic cleaner for 5 minutes. Weigh 185-259mg of ammonium fluoride and dissolve it in 2ml of water, and add 5ml of absolute ethanol; add the ammonium fluoride solution to the above solution, ultrasonically in an ultrasonic cleaner for 5 minutes to mix the two solutions evenly, and dissolve the solution with nitric acid and ammonia water The pH value of the solution is adjust...

Embodiment 2

[0052] Example 2. Rare earth fluoride nanomaterials used as MALDI-MS matrix for mass spectrometric analysis of organic bases

[0053] The preparation of rare earth ytterbium fluoride nanomaterial is the same as in Example 1

[0054] The application process of rare earth fluoride nanomaterials as a matrix in MALDI-MS is as follows:

[0055] Step 1. Weigh 1 mg of ytterbium trifluoride hollow nanospheres, add 1 to 2 ml of water, and ultrasonically disperse for 1 to 2 minutes to prepare a matrix solution;

[0056] Step 2. preparation concentration is the 2-phenylbenzimidazole solution of 1mM;

[0057] Step 3. Take 1-2 μL of 2-phenylbenzimidazole solution and spot it on the surface of the metal target, and form a thin layer of the sample after natural drying;

[0058] Step 4. Take 1-2 μL of matrix solution and spot on the thin layer of the sample. After natural drying, mass spectrometry analysis can be performed. The results are shown in figure 2 .

[0059] Yttrium trifluoride...

Embodiment 3

[0060] Example 3. Rare earth fluoride nanomaterials are used as a MALDI-MS matrix for mass spectrometry analysis of diantipyrine methane

[0061] The preparation of rare earth praseodymium fluoride nanomaterial is the same as in Example 1

[0062] The application process of rare earth fluoride nanomaterials as a matrix in MALDI-MS is as follows:

[0063] Step 1. Weigh 1 mg of praseodymium trifluoride nanoparticles, add 1 to 2 ml of methanol, and ultrasonically disperse for 1 to 2 minutes to prepare a matrix solution;

[0064] Step 2. preparation concentration is the diantipyrine methane solution of 1mM;

[0065] Step 3. Take 1-2 μL of diantipyrine methane solution and spot on the surface of the metal target, and form a thin layer of the sample after natural drying;

[0066] Step 4. Take 1-2 μL of matrix solution and spot on the thin layer of the sample. After natural drying, mass spectrometry analysis can be performed. The results are shown in image 3 .

[0067] Yttrium t...

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Abstract

The invention relates to a matrix material for MALDI, in particular to application of fluoride nanometer materials as a matrix in MALDI-MS. The fluoride nanometer materials are nano-particles with various shapes of ytterbium trifluoride, lanthanum trifluoride, cerium trifluoride, praseodymium trifluoride, neodymium trifluoride, samarium trifluoride, europium trifluoride, gadolinium trifluoride, terbium trifluoride, dysprosium trifluoride, holmium trifluoride, erbium trifluoride, thulium trifluoride, ytterbium trifluoride or lutetium trifluoride, or nano-particles of trifluorides compounded byany two or more of ytterbium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium or lutetium in any proportion. The invention takes rare earth fluoride nanometer materials as the matrix of the MALDI-MS for the first time, develops the matrix of the MALDI-MS with simple and convenient operation and strong universality, and realizes the quick and accurate analysis of the MALDI-MS.

Description

technical field [0001] The invention relates to a matrix material for matrix-assisted laser desorption ionization (MALDI), in particular to the application of a rare earth fluoride nanometer material as a MALDI matrix in MALDI-MS. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a new type of soft ionization biological mass spectrometry developed in recent years. Its working principle is: when the laser irradiates the co-crystal mode formed by the sample and the matrix, the matrix absorbs energy from the laser and transmits it to the sample molecules, ionizing the sample molecules to form sample ions; the charged sample ions are accelerated under the action of an electric field Fly through the flight tube, arrive at the detector, and be detected according to the flight time of the detector, that is, the mass-to-charge ratio (M / Z) of the measured ion is proportional to the flight time of the ion, and ...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 王志林陈志明耿志荣
Owner NANJING UNIV
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