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Application of acidic buffer in stable pollen allergen activity

A technology of acidic buffer and citrate buffer, applied in the field of biopharmaceuticals, can solve the problems of complex composition and structure, the inability of pollen allergen drugs to be stored for a long time at room temperature, and the reduction of potency

Active Publication Date: 2009-08-05
ZHEJIANG WOLWO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex composition and structure of pollen allergens, they are easily affected by physical or chemical factors to lose their activity and reduce their potency.
Therefore, in order to ensure the curative effect of pollen allergen drugs used for specific immunotherapy, pollen allergen drugs generally cannot be stored at room temperature for a long time, and their transportation must also be carried out in a low temperature environment, which poses challenges for storage, use and transportation. caused great inconvenience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of mugwort pollen allergen extracts with different pH values

[0056] (1) Artemisia mugwort pollen is dried naturally to constant weight;

[0057] (2) Degrease with acetone several times until the acetone layer is colorless;

[0058] (3) After the acetone is completely volatilized, the sample is pulverized by a high-speed disperser (3000g, 30 times, 5s / time), and the above operations are all carried out at 4°C;

[0059] (4) Add 10ml coca’s (5.0g sodium chloride, 2.75g sodium bicarbonate, add distilled water to 1000ml) per gram of pollen, soak at 4°C for 48 hours, stir with a magnetic stirrer several times during this period, 20 minutes each time;

[0060] (5) After leaching, centrifuge to get the supernatant to obtain the crude extract;

[0061] (6) Filter successively with 0.45 μm and 0.22 μm filter membranes to remove small insoluble particles;

[0062] (7) Concentration: The above-mentioned filtered extract is ultrafiltered with an ultrafiltr...

Embodiment 2

[0064] Example 2 Preparation of Humulus pollen allergen extracts with different pH values

[0065] (1) Humulus pollen is naturally dried to constant weight;

[0066] (2) Degrease with acetone several times until the acetone layer is colorless;

[0067] (3) After the acetone is completely volatilized, the sample is pulverized by a high-speed disperser (3000g, 30 times, 5s / time), and the above operations are all carried out at 4°C;

[0068] (4) Add 10ml coca’s (5.0g sodium chloride, 2.75g sodium bicarbonate, add distilled water to 1000ml) per gram of pollen, soak at 4°C for 48 hours, stir with a magnetic stirrer several times during this period, 20 minutes each time;

[0069] (5) After leaching, centrifuge to get the supernatant to obtain the crude extract;

[0070] (6) Filter successively with 0.45 μm and 0.22 μm filter membranes to remove small insoluble particles;

[0071] (7) Concentration: The above-mentioned filtered extract is ultrafiltered with an ultrafiltration memb...

Embodiment 3

[0073] Example 3 Preparation of allergen extracts from sycamore pollen with different pH values

[0074] (1) French sycamore pollen is naturally dried to constant weight;

[0075] (2) Degrease with acetone several times until the acetone layer is colorless;

[0076] (3) After the acetone is completely volatilized, the sample is pulverized by a high-speed disperser (3000g, 30 times, 5s / time), and the above operations are all carried out at 4°C;

[0077] (4) Add 10ml coca’s (5.0g sodium chloride, 2.75g sodium bicarbonate, add distilled water to 1000ml) per gram of pollen, soak at 4°C for 48 hours, stir with a magnetic stirrer several times during this period, 20 minutes each time;

[0078] (5) After leaching, centrifuge to get the supernatant to obtain the crude extract;

[0079] (6) Filter successively with 0.45 μm and 0.22 μm filter membranes to remove small insoluble particles;

[0080] (7) Concentration: the leaching liquid after above-mentioned filtering is carried out u...

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PUM

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Abstract

The invention discloses application of an acid buffer solution with the pH value of between 3.0 and 6.0 (the preferred pH value is between 4.0 and 5.0) in stabilizing the activity of pollen allergen. The invention also discloses a pharmaceutical composition, which contains at least one allergen from pollen, the acid buffer solution with the pH value of buffer effective amount of between 3.0 and 6.0, and a pharmaceutically acceptable carrier. The pharmaceutical composition can be stored, used and transported at normal temperature.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to the application of an acid buffer solution in stabilizing pollen allergen activity for a long time under normal temperature conditions. Background technique [0002] Allergic disease is one of the most common and stubborn diseases in the world today, and it is a disease that seriously threatens human health. The incidence of allergic diseases accounts for about 20% of the population, and may occur in all age groups from newborns to middle-aged and elderly people. There is no obvious gender tendency, but there is an obvious genetic tendency. Anaphylaxis is a common allergic reaction, mainly for respiratory allergic reaction, gastrointestinal allergic reaction, skin allergic reaction and anaphylactic shock. Symptoms mainly include allergic rhinitis, allergic asthma, allergic gastroenteritis, and allergic skin diseases such as eczema, urticaria, macule, papul...

Claims

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Application Information

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IPC IPC(8): A61K47/12A61K39/00A61K9/08A61K9/10A61K9/12A61K9/72A61P37/08A61K47/02
Inventor 秦承学
Owner ZHEJIANG WOLWO BIOTECH
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