Reagent kit for quantitatively detecting enrofloxacin content in food and its use
A technology for enrofloxacin and quantitative detection, which is applied in measurement devices, analysis by chemical reaction of materials, instruments, etc., can solve the problems of stability, poor repeatability, product residue and influence, etc., and achieve high stability. , easy-to-save effects
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[0054] a. Pretreatment of samples to be tested: according to different types of samples to be tested, the following processing methods are adopted respectively;
[0055] 1. Pork and pork liver, beef, chicken and its products, fish, squid, shrimp (dilution factor: 50)
[0056] Weigh 1 g of the homogenized sample, put it in a 50 ml polystyrene centrifuge tube, add 4 ml of 70% methanol solution to the sample, mix with a vortex mixer for 10 minutes, centrifuge at 4000 rpm at 15°C for 10 minutes. The supernatant was diluted 10 times with the extract for detection.
[0057] 2. Milk (dilution factor: 100)
[0058]Add 9ml of 70wt% methanol to 1ml of sample, mix with a vortex mixer for 10 minutes, centrifuge at 4000 rpm at 15°C for 10 minutes. The supernatant was diluted 10 times with the extract for detection.
[0059] 3. Honey (dilution factor: 100)
[0060] Weigh 1g of honey into a 50ml polystyrene centrifuge tube, add 2ml of 0.02M PB, shake fully until the honey is completely d...
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