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Determination kit for triazophos chemoluminescence immunoassay and preparation method and using method thereof

A technology of triazophos chemistry and luminescent immunity, which is applied in the direction of analytical materials, measuring devices, scientific instruments, etc., can solve the problems of high minimum detection limit of triazophos and cannot fully meet the detection requirements, and achieve the effect of high sensitivity

Active Publication Date: 2011-09-07
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problem that the existing direct competitive enzyme-linked immunosorbent assay method has a high minimum detection limit for triazophos and cannot fully meet the detection requirements, and provides a triazophos chemiluminescence immunoassay assay kit and its preparation and how to use

Method used

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  • Determination kit for triazophos chemoluminescence immunoassay and preparation method and using method thereof
  • Determination kit for triazophos chemoluminescence immunoassay and preparation method and using method thereof

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specific Embodiment approach 1

[0012]Specific embodiment one: the triazophos chemiluminescent immunoassay assay kit of this embodiment consists of a triazophos standard, a solid phase carrier coated with a triazophos monoclonal antibody, and a triazophos labeled with horseradish peroxidase Hapten, chemiluminescent sensitizer, and PBST buffer solution with a pH value of 7.4 at a concentration of 0.01mol / L; the triazophos standard consists of a blank sample and a triazophos concentration from 0.01ng / mL to 2.5 ng / mL selected 6-8 values ​​of the sample composition; chemiluminescence sensitization solution is based on the concentration of chemiluminescence substrate is 0.5mmol / L ~ 3mmol / L, the concentration of p-iodophenol is 0.1mmol / L ~ 1mmol / L, the concentration of hydrogen peroxide is 1mmol / L~10mmol / L, and the solution prepared by diluting Tris-HCl buffer solution with a concentration of 0.1mol / L and a pH value of 8.5, wherein the chemiluminescence substrate is Luminol, isoluminol, or 7-amino-6-mercaptophtha...

specific Embodiment approach 2

[0015] Embodiment 2: This embodiment is different from Embodiment 1 in that the solid phase carrier is a microporous plate, plastic beads, plastic tubes or magnetic particles. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the concentrations of triazophos in the standard triazophos are 7 values ​​selected from 0.02 ng / mL to 2.4 ng / mL. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a preparation method of a determination kit for triazophos chemoluminescence immunoassay and relates to determination kits for triazophos chemoluminescence immunoassay and a preparation method and a using method thereof. The invention solves the technical problem of high minimum detection limit in the conventional direct competitive enzyme-linked immunosorbent assay method. The determination kit for triazophos chemoluminescence immunoassay consists of a triazophos standard substance, a carrier coating a triazophos monoclonal antibody, horse radish peroxidase-labeled triazophos hapten, a chemiluminescent sensitizing solution and phosphate buffer saline (PBS). The kit is prepared by putting the prepared triazophos standard substance, the carrier coating the triazophos monoclonal antibody, the chemiluminescent sensitizing solution and the PBS buffer solution into a box to obtain the determination kit. When the kit is used, the triazophosstandard substance and samples are added into carrier reactive pores respectively and washed, and the chemiluminescent sensitizing solution is added, then the luminescent intensity is determined and specification curve is drawn, and finally the concentration of triazophos in the sample is determined. The minimum detection limit of triazophos is 0.06ng / mL, so the invention can be used in the field of foods.

Description

technical field [0001] The invention relates to a triazophos analysis and determination kit and a preparation and use method thereof. Background technique [0002] With the gradual withdrawal of highly toxic pesticides from the market, triazophos has become the main pesticide to replace methamidophos, and is widely used in the control of rice stem borer in the middle and lower reaches of the Yangtze River. In recent years, its use has increased rapidly. While triazophos pesticide is entering the peak season of the market in my country, foreign countries have begun to restrict or prohibit the use and sales of triazophos pesticide. On November 28, 2004, the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China issued an emergency warning, saying that the European Union would officially ban the sale of 320 pesticides in the European Union starting from December 31. It involves more than 60 varieties of pesticides being produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/545G01N33/546G01N33/535
Inventor 金茂俊王静邵华金芬
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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