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Shuang Huanglian liquid preparation and method for measuring content thereof

A technology of liquid preparations and measurement methods, which is applied in the direction of anti-inflammatory agents, antiviral agents, and measuring devices, which can solve problems such as prolonging detection time, increasing detection costs, and increasing detection workload, so as to improve detection efficiency and reduce detection costs , reduce the effect of allergic reactions

Active Publication Date: 2009-07-08
HEILONGJIANG ZBD PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that in the prior art, in order to detect the content of chlorogenic acid, forsythin and baicalin, three sets of different chromatographic conditions are used, which obviously increases the detection workload, prolongs the detection time, and increases the detection cost
In addition, the detection methods in the prior art usually only detect the content of baicalin, forsythin and chlorogenic acid, but do not detect the content of the other two active ingredients luteolin and wogonin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Preparation of honeysuckle extract

[0018] Weigh honeysuckle, soak in water for 2-3 times, filter, concentrate, ethanol precipitation, filter, recover ethanol from the filtrate until it has no ethanol smell, add purified water 2-6 times the paste weight, stir gently, let stand, take The supernatant was filtered, and the filtrate was concentrated, then alcohol-precipitated again, allowed to stand, and the supernatant was filtered and concentrated to obtain the honeysuckle extract.

[0019] Using high performance liquid chromatography to detect, it is measured that the extract contains 15.22 mg / g of chlorogenic acid and 0.38 mg / g of luteolin.

[0020] 2) Preparation of Forsythia Extract

[0021] Weigh the forsythia, decoct it with water for 3 times, combine the liquid medicine, and follow up with the steps after warming the honeysuckle to obtain the forsythia extract.

[0022] It is detected by high performance liquid chromatography, and the extract contains 4.05mg / ...

Embodiment 2

[0027] Prepare following formula with 3 kinds of extracts that obtain in embodiment 1:

[0028] Preparation of injection (the volume of each injection is 10ml)

[0029] 1) Formula 1: chlorogenic acid 0.4mg / ml, baicalin 6.0mg / ml, forsythin 0.1mg / ml, wogonin 0.09mg / ml, luteolin 0.01mg / ml.

[0030] 2) Formula 2: chlorogenic acid 1.0 mg / ml, baicalin 10.0 mg / ml, forsythin 0.8 mg / ml, wogonin 0.15 mg / ml, luteolin 0.024 mg / ml.

[0031] 3) Formula 3: chlorogenic acid 1.5 mg / ml, baicalin 9.0 mg / ml, forsythin 0.3 mg / ml, wogonin 0.13 mg / ml, luteolin 0.04 mg / ml.

[0032] 4) Formula 4: chlorogenic acid 2.0 mg / ml, baicalin 8.0 mg / ml, forsythin 0.5 mg / ml, wogonin 0.12 mg / ml, luteolin 0.05 mg / ml.

[0033] The preparation of oral liquid (the filling capacity of each oral liquid is 10ml)

[0034] 5) Formula 5: chlorogenic acid 0.6 mg / ml, baicalin 8.0 mg / ml, forsythin 0.3 mg / ml, wogonin 0.12 mg / ml, luteolin 0.015 mg / ml.

[0035] 6) Formula 6: chlorogenic acid 1.0 mg / ml, baicalin 12.0 mg / ml, f...

Embodiment 3

[0039] Protective Effect of Shuanghuanglian Liquid Preparation on Influenza Virus Infection in Mice

[0040] Medicine: Shuanghuanglian injection of the present invention (formulation 1, new Shuanghuanglian 1): chlorogenic acid 0.4mg / ml, baicalin 6.0mg / ml, forsythin 0.1mg / ml, wogonin 0.09mg / ml, Luteolin 0.01mg / ml.

[0041] Shuanghuanglian oral liquid of the present invention (formulation 5, new Shuanghuanglian 2): chlorogenic acid 0.6mg / ml, baicalin 8.0mg / ml, forsythin 0.3mg / ml, wogonin 0.12mg / ml, luteolin Glycoside 0.015mg / ml.

[0042] Commercially available Shuanghuanglian injection: chlorogenic acid 1.3mg / ml, baicalin 7.2mg / ml, forsythin 0.04mg / ml, wogonin 0.02mg / ml, luteolin 0.003mg / ml. .

[0043] Commercially available Shuanghuanglian oral liquid: chlorogenic acid 1.1mg / ml, baicalin 8.4mg / ml, forsythin 0.4mg / ml, wogonin 0.04mg / ml, luteolin 0.002mg / ml.

[0044] Animals: ICR closed group mice, body weight (20±2) g, half male and half male

[0045] Virus strain: Influenz...

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Abstract

The invention mainly relates to a Shuanghuanglian liquid preparation and a method for measuring the content thereof. The Shuanghuanglian liquid preparation mainly comprises scutelloside, forsythin, chlorogenic acid, and the like, and the adverse reaction thereof is mainly shown in anaphylactic shock, erubescent skin and exanthemata, etc. The invention provides the Shuanghuanglian liquid preparation containing proper chlorogenic acid, scutelloside, forsythin and other effective components, and not only has ideal therapeutic effect, but also reduces the occurrence of the anaphylactic reaction. The invention also provides the method for measuring the content of the Shuanghuanglian liquid preparation; during the application of the measurement method of the invention, only one moving phase is needed to simultaneously measure the content of the five effective components, i.e. the scutelloside, the forsythin, the chlorogenic acid, luteoloside and wogonin. The method not only obviously improves the measurement efficiency, but also effectively reduces the measurement cost.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a Shuanghuanglian liquid preparation and a content determination method thereof. Background technique [0002] Shuanghuanglian liquid preparation is extracted and refined from honeysuckle, forsythia and scutellaria baicalensis, and mainly contains baicalin, forsythin, chlorogenic acid, luteolin, wogonin, etc. Modern pharmacological studies have proved that Shuanghuanglian liquid preparation has antibacterial, antiviral, and antipyretic effects. Clinically, it is widely used in the treatment of upper respiratory tract infection, pneumonia, tonsillitis, pharyngitis, etc. caused by viral and bacterial infections. However, with the widespread clinical application of Shuanghuanglian liquid preparations, reports of adverse reactions are common. The adverse reactions of Shuanghuanglian liquid preparation mainly include anaphylactic shock, skin flushing, and ras...

Claims

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Application Information

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IPC IPC(8): A61K36/634A61P31/04A61P31/12A61P29/00A61P11/00G01N30/36
Inventor 方同华项彦华岳大彪周雪峰王春生周广红
Owner HEILONGJIANG ZBD PHARMA
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