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Identification plate for gram negative aerobic bacteria and preparation thereof

A technology for identifying plates and aerobic bacteria, which is applied in biochemical equipment and methods, microbiological measurement/inspection, specific-purpose bioreactors/fermenters, etc., and can solve problems such as long identification time and narrow identification range

Inactive Publication Date: 2009-07-01
上海星佰生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The identification time is too long, and it takes 16-24 hours to give the result;
[0008] (2) The scope of identification is relatively narrow, and there are only more than 300 kinds of bacteria that can be identified

Method used

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  • Identification plate for gram negative aerobic bacteria and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0072] Gram-negative aerobic bacteria identification plate, the production process of batch production of 375 products is as follows:

[0073] 1. Configure the identification plate reaction substrate stock solution

[0074] hole position drug name The amount of pure water needed in this batch (ml) The amount of drug required in this batch (g) A1, A7 water 17 17 A2, A8 Tween 80 17 1.02 A3, A9 N-acetyl-D-galactosamine 17 0.34 A4, A10 L-Arabinose 17 0.68 A5, A11 D-Arabitol 17 0.68 A6, A12 D-cellobiose 17 0.68 B1, B7 D-fructose 17 1.36 B2, B8 D-galactose 17 0.68 B3, B9 α-D-glucose 17 1.7 B4, B10 glycerin 17 0.34 B5, B11 α-D-lactose 17 0.68 B6, B12 maltose 17 0.68 C1, C7 D-Mannitol 17 1.36

[0075] C2, C8 D-melibiose 17 0.68 C3, C9 β-methyl-D-glycoside) 17 0.68 C4, C10 D-Allulose 17 0.68 C5, C11 D-Raffinose- ...

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PUM

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Abstract

The invention provides an identification plate for Gram-negative aerobic bacteria. The combination of 47 carbon sources and nitrogen sources on the identification plate can identify more than 300 kinds of Gram-negative bacteria, can identify various bacteria such as negative Enterobacteriaceae, Vibrionaceae, pseudomonas, Acinetobacter, stenotrophomonas, negative fastidious bacteria, and so on, can obtain an identification result without a supplementary experiment, and has simple operation and accurate results, so the identification plate has higher clinical application value. The invention provides a method for preparing the identification plate for the Gram-negative aerobic bacteria.

Description

Technical field: [0001] The present invention relates to biological agents. In particular, it relates to a Gram-negative aerobic bacteria identification board and a preparation method thereof. Background technique [0002] In the past 20 years, due to the widespread use of a wide variety of antibacterial drugs in clinical practice, drug resistance of bacteria has become more and more common. Some bacteria can be resistant to 2-7 kinds of drugs at the same time. Very difficult, especially for nosocomial bacteria. The characteristic of nosocomial infection is that it occurs in the hospital environment and is popular, so it can also be called hospital acquired infection (Hospital acquired infection). Although the conditions of nosocomial infection vary from mild to severe, they all add extra pain, economic burden and misfortune to patients. At the same time, they will increase the burden of medical and nursing work due to prolonged hospitalization, and affect the turnover of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12M1/34
Inventor 邹艳芳陈弛宇朱骏娜
Owner 上海星佰生物技术有限公司
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