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Preparation of quantitative rapid detecting sensor of microcapsule algae toxin and applications

A detection sensor, microcystin technology, applied in the biological field, can solve the problems of time-consuming and laborious, unable to monitor the source water in real time, and achieve the effect of sensitive detection

Inactive Publication Date: 2012-01-25
上海雄图生物科技有限公司
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AI Technical Summary

Problems solved by technology

However, these methods are time-consuming and laborious, have hysteresis, and cannot monitor the source water in real time.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Quantitative and rapid detection of microcystin-LR in natural lake water:

[0030] 1 Pre-processing:

[0031] Sampling was carried out by a plexiglass deep-water sampler, the water sampling depth was below 0.5m, and the sampling volume was 5L. Remove large particles of impurities through a sand core funnel, and then filter under reduced pressure through a 0.45 μm filter membrane. Collect the filtrate. Take an appropriate amount of filtrate for detection and analysis.

[0032] 2. Microcystin-LR quantitative rapid detection sensor usage method:

[0033] Take out the prepared MC-LR immune electrode sensor and equilibrate to room temperature. In order of concentration from low to high, 10 μL of MC-LR standard solution and sample extract of different concentrations were added dropwise on the surface of the electrode, incubated at 37°C for 15 min, washed with PBS and dried. Using cyclic voltammetry (CV), alternating current impedance method (EIS) in [Fe(CN)6] ...

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Abstract

The invention provides preparation and application of a quantitative and rapid detection biosensor of microcystic toxin-LR, and belongs to the biotechnology field. The detection sensor is an impedance type electrochemical sensor, and consists of a CHI760C type electrochemical work station and a three-electrode system in which an immunity electrode is taken as a working electrode, a saturated calomel electrode is taken as a reference electrode and a platinum wire electrode is taken as a counter electrode; L-cysteine, colloidal gold and microcystic toxin antibody compound are respectively enveloped on a surface of a bare mitallic electrode to be taken as the working electrode; a change of electric potential difference between the reference electrode and the working electrode takes place when the microcystic toxin antibody is combined with the antigen (MC-LR) in sample solution, and is subject to a quantitative relation with the concentration of the antigen in the sample, then the detection of the microcystic toxin is carried out; the biosensor for rapidly, specifically and sensitively detecting microcystic toxin antigens is successfully established; the detection linear range is 0.05-300ng / mL, and the detection limit is 1.82*10<-2>ng / mL. The biosensor can perform real-time monitoring on natural lake water, and can also perform real-time monitoring on preparation process of drinking water in water supply works.

Description

technical field [0001] The preparation and application of a microcystin-LR quantitative rapid detection sensor belongs to the field of biotechnology. Background technique [0002] Microcystins (MC for short) have become one of the pollutants that are widely concerned in environmental water. MC is a kind of biologically active cyclic heptapeptide compound, which is mainly produced by the freshwater alga Microcystis aeruginosa. So far, more than 70 isomers of MC have been found. It is a highly specific toxin with the liver as the target organ, and has obvious hepatotoxicity. With the in-depth study of MC toxicity, it is found that MC has multi-organ toxicity, hereditary and carcinogenicity. The structure of algal toxins is mainly composed of N-methyl dehydroalanine and two L-amino acid residues X and Z. Common three toxins are MC-LR, RR, and YR (L, R, and Y represent leucine respectively Acid, arginine, tyrosine), and MC-LR is the main toxin in fresh water. Algae toxin is a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/543
Inventor 孙秀兰张银志石慧肖理文李鲁宁许欣徐丹
Owner 上海雄图生物科技有限公司
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