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Rice protein OsSRM and coding gene and application thereof

A rice protein and coding technology, which is applied in the fields of application, genetic engineering, and plant gene improvement, can solve the problems of unknown rice function impact and gene function, etc., and achieve the effects of increasing farmers' income, improving yield and quality, and increasing yield

Inactive Publication Date: 2009-06-17
HANGZHOU ACAD OF AGRI SCI
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Problems solved by technology

[0005] As we all know, the genetic map of rice has been drawn, which means that most of the rice gene sequences are public, but the specific function of a certain gene is unknown, especially the protein encoded by a certain gene and its effect on rice production. The functional impact of the

Method used

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  • Rice protein OsSRM and coding gene and application thereof
  • Rice protein OsSRM and coding gene and application thereof
  • Rice protein OsSRM and coding gene and application thereof

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Embodiment Construction

[0015] Gene acquisition

[0016] (1) The hybrid rice salt-tolerant combination Shanyou No. 10 and the salt-sensitive combination Liangyoupeijiu were used as materials, and their seeds were sown on filter paper culture dishes soaked in 100mM NaCl solution, placed in an incubator at 30°C to germinate, and the salt was changed every day. solution to keep the salt concentration essentially constant. After the seedlings grew to 10 days, the leaves of Shanyou 10 and Liangyoupeijiu seedlings were collected respectively.

[0017] (2) Precipitation with cold acetone / trichloroacetic acid (Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J.A proteomic approach to analyzing drought-andsalt-responsiveness in rice. Field Crop Res, 2002, 76(2-3 ): 199-219) to quickly extract total leaf protein, the specific operation is as follows:

[0018] 1) Rice leaves are ground into fine powder with liquid nitrogen, put into 1.5ml centrifuge tubes, add 1ml protein extract solution I (acetone...

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Abstract

The invention discloses a paddy salt tolerant protein OsSRM. The protein has an ammonia acid sequence shown in the sequence list SEQ ID NO.1. The protein which has excessive expression in paddy seedlings can improve the high salt and high temperature tolerance of the paddy seedlings. If the gene coding protein is converted into the plants of vegetables and flowers such as Arabidopsis thaliana, capsicum, eggplant, strawberry, scarlet sage, flameray gerbera, and the like, the protein can improve the high salt and high temperature tolerance of the plants, help increase the yield of paddy, open field vegetables and flowers in the alkaline land, and improve the utilization of the alkaline land in the coastal areas of the country. The protein can overcome the continuous cropping barriers caused by soil salt return or high temperature under the current facility conditions, improve the yield and quality of the plants of vegetables and flowers, and promote agricultural efficiency improvement and farmer income increase.

Description

technical field [0001] The invention relates to the field of functional genomics, in particular to a rice protein OsSRM and its coding gene and application. Background technique [0002] The threat of soil salinization to agriculture is a global problem. There are a total of 1 billion hectares of saline-alkali land in the world, accounting for about 7.6% of the world's land area. my country's saline-alkali land is nearly 100 million hectares, and nearly 3.335 million hectares of agricultural land has been reduced or abandoned due to salinization. In recent years, my country's facility agriculture has developed rapidly, especially the production area of ​​vegetable and flower greenhouses has continued to expand. According to statistics, in 2005, the facility cultivation area of ​​vegetables, flowers, melons and fruits in China reached 2.1 million hectares. The development of facility agriculture has played an important role in the adjustment of agricultural production struct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29A01H5/00
Inventor 阮松林马华升王世恒童建新忻雅王淑珍来文国郑桂珍何俊平白宇杰
Owner HANGZHOU ACAD OF AGRI SCI
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