Papaya anti-oxidant serum and preparation method thereof
An anti-oxidant and essence technology, which is applied in the directions of antitoxins, pharmaceutical formulations, and medical preparations with inactive ingredients, etc., can solve problems such as no research reports, and achieve high extraction efficiency, mild reaction conditions and large extraction volume Effect
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Embodiment 1
[0032] Embodiment 1, the preparation of papaya antioxidant essence
[0033] Include the following steps:
[0034] a. Pulping: Wash the fresh papaya, remove the core, and cut into small pieces (1cm 3 ), add water according to the weight ratio of papaya and water as 1:0.5, and grind to obtain pulp;
[0035] B, enzymatic hydrolysis: in the papaya pulp obtained in step a, the hydrolytic enzyme consisting of pectinase and cellulase (weight ratio of 1: 1) is added by the weight ratio of papaya pulp and hydrolytic enzyme as 100:0.2 Enzyme, the enzymolysis reaction is carried out under the conditions of a temperature of 30°C, a pressure of -0.11Mpa, and a stirring speed of 180 rpm for 90 minutes to obtain papaya enzymolysis pulp;
[0036] c. Filtration: filter the papaya enzymatic pulp obtained in step b to 100 meshes, collect the filtrate and filter residue respectively, and the filtrate is the papaya enzymatic water extract;
[0037] d. Alcohol extraction: in the filter residue o...
Embodiment 2
[0041] Embodiment 2, the preparation of papaya antioxidant essence
[0042] Include the following steps:
[0043] a. Pulping: Wash the fresh papaya, remove the core, and cut into small pieces (1cm 3 ), add water according to the weight ratio of papaya and water as 1:1, and grind to obtain pulp;
[0044] B, enzymatic hydrolysis: in the papaya pulp obtained in step a, add cellulase in the weight ratio of papaya pulp and cellulase as 100:6, at a temperature of 40°C, a pressure of -0.10Mpa, and a stirring speed Carry out the enzymatic hydrolysis reaction under the condition of 220 rpm for 70 minutes to obtain papaya enzymatic hydrolysis pulp;
[0045] c. Filtration: filter the papaya enzymatic pulp obtained in step b to 100 meshes, collect the filtrate and filter residue respectively, and the filtrate is the papaya enzymatic water extract;
[0046] d. Alcohol extraction: in the filter residue obtained in step c, add an ethanol solution with a volume percentage concentration of ...
Embodiment 3
[0050] Embodiment 3, the preparation of papaya antioxidant essence
[0051] Include the following steps:
[0052] a. Pulping: Wash the fresh papaya, remove the core, and cut into small pieces (1cm 3 ), add water according to the weight ratio of papaya and water as 1:2, and grind to obtain pulp;
[0053] B, enzymatic hydrolysis: in the papaya pulp obtained in step a, the hydrolytic enzyme consisting of pectinase and cellulase (weight ratio of 1: 1) is added by the weight ratio of papaya pulp and hydrolytic enzyme to 100:2. Enzyme, the enzymolysis reaction is carried out under the conditions of a temperature of 40°C, a pressure of -0.09Mpa, and a stirring speed of 200 rpm for 60 minutes to obtain papaya enzymolysis pulp;
[0054] c. Filtration: filter the papaya enzymatic pulp obtained in step b to 100 meshes, collect the filtrate and filter residue respectively, and the filtrate is the papaya enzymatic water extract;
[0055] d. Alcohol extraction: in the filter residue obta...
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