Application of rosmarinic acid in bacillus coli L-asparaginase activator

Abstract

The invention relates to application of rosmarinic acid in an escherichia coli L-asparaginase enzyme activator, which belongs to the technical field of biomedicine. The application comprises the following steps: firstly, preparing an L-asparaginase enzyme extract from escherichia coli; secondly, adding the rosmarinic acid and an L-asparaginase which have different concentrations into an enzyme activity measuring system of the L-asparaginase respectively; thirdly, measuring the reaction speed of each reaction system, calculating the michaelis constant under the concentration of each rosmarinic acid, and judging the activation of the michaelis constant on the L-asparaginase enzyme. The rosmarinic acid can significantly improve the degradation activity of the L-asparaginase enzyme to catalyze the L-asparagine when the concentration of the rosmarinic acid is between 0.375 x 10<-4> and 3.0 x 10<-4> g / L. The application can reduce the dosage of the L-asparaginase enzyme in the process of tumor therapy clinically, and reduce side effects caused by immunogenicity of the L-asparaginase enzyme, or a rosmarinic acid derivative is designed aiming at a target point of the bacterium L-asparaginase enzyme and is used as a new antibacterial drug.

Description

Technical field: [0001] The invention relates to the application of rosmarinic acid in Escherichia coli L-asparaginase activator and belongs to the field of biomedicine. Background technique: [0002] L-Asparaginase (L-Asparaginase; EC3.5.1.1), also known as L-asparaginase or L-asparaginase, trade name L-asparaginase, is a kind of anticancer activity Enzyme protein, widely found in animal tissues, bacteria, plants and the serum of some rodents, in Escherichia coli as an intracellular enzyme, but its distribution in various tissues and organs of the human body has not been reported, originally from guinea pig serum extracted from. The active form of L-asparaginase is a homologous tetramer, each subunit is composed of 330 amino acids, which can specifically catalyze the hydrolysis of L-asparagine into aspartic acid and ammonia. [0003] In 1953, Kidd found that guinea pig serum had an anticancer effect. In 1961, Broom confirmed that the antitumor factor in guinea pig serum w...

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Problems solved by technology

[0006] L-asparaginase is often derived from Escherichia coli. As a heterologous protein of the human body, clinical use often causes some side effects, ranging from mild allergic reactions such as fever, nausea, vomiting, diarrhea, etc. to dangerous anaphylactic shock and thromboembolism, Pancreatitis, etc., which is related to the immune response caused by bacterial proteins, this adverse reaction limits the therapeutic application of L-asparaginase

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