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Method for technical evaluation of hedgehog fungus Houjie bacterial with numerator mark

A technology of molecular markers and technical identification, which is applied in the field of identification of Hericium erinaceus strains and microorganisms using molecular marker technology, can solve the problems of inconvenience in the production process, scientific research of edible fungi and obstacles in academic exchanges, and achieve the detection time Short, high sensitivity, effect of reducing deviation

Inactive Publication Date: 2009-04-08
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

This kind of confusion and confusion not only brings a lot of inconvenience to the production process, but also creates obstacles to the scientific research and academic exchange of edible fungi.

Method used

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  • Method for technical evaluation of hedgehog fungus Houjie bacterial with numerator mark

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Embodiment 1

[0018] Embodiment 1: A kind of method utilizing molecular marker technology to identify Hericium Hericium Hericium species

[0019] A method for identifying Hericium erinaceus strains using molecular marker technology, comprising the following steps:

[0020] 1. Mycelia culture and collection

[0021] (1) If the preservation time of Hericium erinaceus strain for testing is less than 6 months, the mycelia culture is carried out as follows:

[0022] 1. Use an inoculation rake to crush the PDA medium containing Hericium erinaceus hyphae, and transfer it to a 250ml Erlenmeyer flask containing 100ml PDA liquid medium;

[0023] 2. Place on a shaker at 25°C with a rotation speed of 90-130r / min and cultivate for 7-10 days;

[0024] 3. Filter the cultured mycelia with gauze, rinse with distilled water, and dry with filter paper;

[0025] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0026] (2) If the Hericium erinaceus strain for ...

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Abstract

The invention relates to a method for identifying the houjie strain of hericium erinaceus by making use of a molecular marker technology, which comprises the culture and collection of mycelium, the extraction of genomic DNA, the detection establishment of SCAR-PCR molecular markers and the detection of SCAR-PCR products. The method provided by the invention for identifying the houjie strain of the hericium erinaceus by making use of the molecular marker technology is high in sensitivity and less in the required quantity of DNA. Compared with the conventional morphologic detection, antagonistic test and fruiting test, the method has the advantages of short detection time, high accuracy, easy judgment, good repetition and the like, provides a rapid and effective system of bacteria identification for the utilization of the germplasm resources of the hericium erinaceus and the registration work of new species, and is very important for scientific research and production.

Description

technical field [0001] The invention relates to a method for identifying microorganisms, in particular to a method for identifying strains of Hericium erinaceus by using molecular marker technology, and belongs to the field of biotechnology. Background technique [0002] Hericium erinaceus (Hericium erinaceus) Hericium erinaceus is one of the famous eight mountain delicacies, and it is listed as the four famous dishes together with bird's nest, shark's fin and sea cucumber. Traditional Chinese medicine believes that Hericium erinaceus is flat in nature and sweet in taste, which can help digestion, benefit the five internal organs, moisten the six lungs, and tonify deficiency and damage. It can improve human immunity and is effective for chronic gastritis, duodenal ulcer, and gastric ulcer. It has a good curative effect on various digestive tract diseases and neurasthenia. Hericium erinaceus is rich in nutrition. It is a high-protein, low-fat, high-quality health food rich i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 朱坚江玉姬谢宝贵邓优锦刘新锐
Owner FUJIAN AGRI & FORESTRY UNIV
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