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Lipase inhibitor from natto, preparation method and use thereof

A lipase inhibitor, lipase technology, applied in the direction of protease inhibitors, peptide sources, animal/human peptides, etc., can solve the problems of increased heart rate, increased blood pressure of patients, restrictions and so on

Inactive Publication Date: 2009-04-01
BEIJING WBL PEKING UNIV BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sibutramine is a monoamine neurotransmitter reuptake inhibitor, it acts on the central nervous system to reduce food intake and reduce body weight, but the drug may cause blood pressure and heart rate to increase in patients, and it cannot be used for coronary heart disease In patients with a history of arrhythmia, stroke, etc., the application of this drug is limited

Method used

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  • Lipase inhibitor from natto, preparation method and use thereof
  • Lipase inhibitor from natto, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Bacillus natto (preserved in the "General Microorganism Center of China Microbial Cultures Preservation Management Committee", the preservation number is CGMCC2186) is activated on the agar slant medium, and the composition of the agar slant medium is (g / L): peptone 10g , beef extract powder 3g, sodium chloride 5g, agar 20g, add distilled water (or deionized water) to 1L, adjust the pH to 7.0, take 4ml and put it in a 15ml test tube, routinely sterilize, the incubation temperature on the slope is 37°C, and the incubation time It was 24 hours; get the slant bacterial classification of above-mentioned activation culture with inoculation loop in the seed medium of sterilization, medium composition is: peptone 10g, beef extract 1g, glucose 5g, (HH 4 ) 2 SO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, K 2 HPO 4 0.2g, add water to 1L, pH7.2, take 100ml and put it into a 500ml shaker flask, plug it with a cotton plug or wrap it with 8 layers of gauze, sterilize it conventionally, cultur...

Embodiment 2

[0096] Bacillus natto (preserved in the "General Microorganism Center of China Microbial Cultures Preservation Management Committee", the preservation number is CGMCC2186) is activated on the agar slant medium, and the composition of the agar slant medium is (g / L): peptone 10g , yeast extract 3g, sodium chloride 10g, agar 20g, add distilled water (or deionized water) to 1L, adjust the pH to 7.0, take 4ml and put it in a 15ml test tube, routinely sterilize, the incubation temperature on the slope is 37°C, and the incubation time It was 22 hours; get the slant bacterial classification of above-mentioned activated culture with inoculation loop in the seed culture medium of sterilization, medium composition is: soybean peptone 10g, yeast extract 1g, glucose 5g, (HH 4 ) 2 SO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, K 2 HPO 4 0.2g, add water to 1L, adjust the pH to 7.0, take 100ml and put it into a 500ml shaker flask, plug it with a cotton plug or wrap it with 8 layers of gauze, and then ...

Embodiment 3

[0098] Bacillus natto (preserved in the "General Microorganism Center of China Microbial Cultures Preservation Management Committee", the preservation number is CGMCC2186) is preserved on the agar slant medium for activation. The composition of the agar slant medium is (g / L): peptone 10g, yeast extract 3g, beef extract 3g, glucose 5g, sodium chloride 5g, agar 20g, add distilled water (or deionized water) to 1L, adjust pH to 7.2, take 5ml and divide into 15ml test tubes, and routinely sterilize , Incline culture temperature 37 ℃, culture time is 22 hours; Get the slant strain of above-mentioned activation culture in the seed culture medium of sterilization with inoculation loop, medium composition is: soybean peptone 10g, beef extract 5g, glucose 5g, ( HH 4 ) 2 SO 4 0.2g, MgSO 4 ·7H 2 O0.2g, K 2 HPO 4 0.2g, add water to 1L, pH 7.0, take 100ml and put it into a 500ml shaker flask, plug it with a cotton plug or wrap it with 8 layers of gauze, then routinely sterilize it, c...

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Abstract

The invention discloses a Bacillus subtilis microorganism, in particular relates to a lipase inhibitor of Bacillus subtilis natto, a preparation method and the function thereof. The lipase inhibitor is prepared by the following steps of: activating and culturing the Bacillus subtilis natto, preparing a component for inhibiting the activity of the lipase by solid fermentation or liquid fermentation, i.e., the culture of natto lipase inhibin, extracting and separating, and eventually preparing the lipase inhibitor. The invention achieves the purpose of reducing weight and fat by inhibiting the activity of the lipase, which has the novelty that: (1) the lipase inhibitor derived from edible natto has no side effects to human body; and (2) the lipase inhibitor selectively acts on gastrointestinal tract lipase, especially on pancreatic lipase without inhibiting prolease, amylase and the like.

Description

technical field [0001] The invention relates to a lipase inhibitor and its application, in particular to a lipase inhibitor derived from natto and its application. Background technique [0002] With the development of economy, the improvement of people's living standard and the change of lifestyle, obesity has become the largest chronic disease among adults in the world. [0003] According to research reports, obesity is directly related to many modern diseases. Obese people have a high risk of cardiovascular and cerebrovascular diseases, diabetes, hypertension, coronary heart disease, gallbladder disease, gout, respiratory diseases, and certain tumors, while hyperlipidemia, visceral Fat is the culprit of many lifestyle-related diseases. Therefore, obesity not only affects people's appearance and body shape, but also seriously threatens human health. [0004] Currently, there are only two drugs approved by the US FDA for long-term obesity treatment: Sibutramine and Orlistat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/81C12N1/20C12P21/02
Inventor 段震文郭树仁何大林闫雪秋
Owner BEIJING WBL PEKING UNIV BIOTECH
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