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Homogeneous allogenic bone transplantation substitute material nano DBM

A technique of allograft and bone transplantation, applied in prosthetics, medical science, etc., can solve the problems of poor biomechanical properties, differences in osteoinductive osteoconduction, failure of bone grafting, etc., to achieve easy material acquisition and no immune rejection , good biocompatibility

Inactive Publication Date: 2009-04-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DBM still has the following disadvantages as a bone graft substitute: 1. There are differences in osteoinductive and osteoconductive properties when processed in batches; 2. Although the antigenicity of DBM is weak, it will show severe immune rejection in a small number of recipients 3. Poor biomechanical properties, not suitable for use in parts that need to bear gravity and other stresses
But so far, there is no report on the successful application of nano-decalcified bone matrix material (nano-DBM) made by nanotechnology for bone grafting.

Method used

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  • Homogeneous allogenic bone transplantation substitute material nano DBM
  • Homogeneous allogenic bone transplantation substitute material nano DBM
  • Homogeneous allogenic bone transplantation substitute material nano DBM

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: prepare rabbit nanometer DBM

[0022] 1. The rabbit DBM bone block was prepared by the modified Urist method, and the steps were as follows:

[0023] 1) Materials: 1 New Zealand rabbit (provided by the Animal Experiment Center of the Second Military Medical University, the same below), 100g of fresh rabbit bone was routinely taken, the periosteum and all soft tissues were removed and split into bone pieces, the bone marrow was scraped off and purified with aseptic Rinse repeatedly with water, dehydrate in absolute ethanol for 2 hours, degrease with ether for 1 hour, and dry overnight in a ventilated place, then freeze-dry the bone in a -80°C refrigerator for later use;

[0024] 2) Preparation of DBM bone blocks:

[0025] (1) Degreasing:

[0026] Soak the above bones in 100ml of absolute ethanol for 2 hours in order to remove the ethanol; add 100ml of ether to soak for 12 hours and remove the ether; rinse with distilled water for 3 times to remove the eth...

Embodiment 2

[0035] Embodiment 2: preparation mouse nanometer DBM

[0036] 20 mice (provided by the Animal Experiment Center of Second Military Medical University, the same below), male or female, weighing 25-30 g. 50 g of bone was routinely taken, and the modified Urist method was used to prepare allogeneic DBM bone blocks and make nano-DBM. The method was the same as in Example 1, and 12 g of nano-DBM was obtained.

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Abstract

The invention relates to the technology field of biological materials and discloses an allogeneic nano-decaicified bone matrix material nano-DBM for bone graft. The nano-DBM is prepared by a fresh allogeneic bone, and the preparation method is as follows: 1) the fresh allogeneic bone is prepared into a DBM bone block by adopting the improved Urist method according to the conventional way; 2) the DBM bone block is arranged in a liquid nitrogen freezing crushing machine for pre-crushing, thereby obtaining DBM powder; 3) the nano-DBM is prepared: the DBM powder is arranged in an ultrafine grinding smashing machine, appropriate distilled water is added, the full grinding is carried out at 17 DEG C to 25 DEG C, homogenate obtained after the grinding is centrifugalized at high speed, supernatant liquid is abandoned, the nano-DBM is obtained, and the nano-DBM is ready for use after shaping and drying. The nano-DBM takes the allogeneic bone as raw materials, the obtainment of the materials is easy; the nano-DBM can be shaped freely; the nano-DBM has good biocompatibility as the bone graft substitute material due to the nano-structure and also has the good ability as a good carrier of exogenous growth factors for promoting the bone regeneration, such as a bone morphogenetic protein, and the like.

Description

technical field [0001] The invention relates to the technical field of biomaterials, and relates to a nano DBM, an allogeneic nano decalcified bone matrix material for bone transplantation. Background technique [0002] Bone transplantation is the most common tissue transplantation after blood transfusion in clinical practice. The repair of bone defects caused by various reasons is a difficult problem for orthopedic surgeons to face. In addition, with the deepening of the understanding of diseases and the development of surgical techniques, the demand for bone transplantation in spine surgery, neurosurgery, etc. is also increasing. [0003] In the past 10 years, the research on bone graft substitutes has been one of the hot spots in the field of spinal surgery. Currently used bone graft substitute materials mainly include autologous bone, allogeneic bone and synthetic materials. An ideal bone graft should have the following three basic characteristics: 1. Contain osteoindu...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 陈雄生朱巍严望军邵将钱列黄凯房雷贾连顺
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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