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Method for obtaining haploid plant of calla by anther culture

A technology of single calla lily and calla lily, applied in the directions of botanical equipment and methods, horticultural methods, plant cells, etc., can solve problems such as no public reports, and achieve the effect of accelerating the process of purification and breeding

Inactive Publication Date: 2010-12-08
YUNNAN UNIV
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Problems solved by technology

[0005] Through document retrieval, do not see the public report identical with the technical content of the present invention

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  • Method for obtaining haploid plant of calla by anther culture

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Embodiment Construction

[0015] 1. Suitable growth conditions for the donor calla lily material.

[0016] The suitable growth season for the donor calla lily material is autumn and winter, and the suitable growth temperature is the highest temperature of 25°C and the lowest temperature of 10°C. For example, the suitable season for open field cultivation of calla lilies in Kunming is autumn (September-November).

[0017] 2. The appropriate development period of anthers and microspores cultured in vitro.

[0018] There is a correlation between the developmental stages of flower buds, anthers and microspores, and the appropriate developmental stage is an important factor affecting the effect of anther culture and the success in obtaining haploid plants of Calla lily. The microspores are in the uninucleate marginal stage, which is the best period for calla lily anther culture. At this time, the tip of the flower bud slightly exposes the petiole, the inflorescence bracts are bright yellow, and the anthers...

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Abstract

The invention relates to a method for obtaining the monoploid plant of Zantedeschia aethiopica by anther culture, belonging to the technical field of flower culture. The method comprises the steps that the young bud of Zantedeschia aethiopica is taken out in the bud stage for sterilization and the anther is taken out and placed on the callus inducement culture medium for culturing and inducing the microspores into callus; the callus is put on the bud inducement culture medium for culturing and inducing the callus to form bud; the bud is put on the radication culture medium for culturing and inducing the bud to radicate and the monoploid plant of Zantedeschia aethiopica is obtained. The method is applied to isolated culture of anther of Zantedeschia aethiopica; on average, 2.4 percent of the anther can induce the microspores to form the callus and each callus can differentiate 6.8 buds and the rate of monoploid plant is 69.6 percent. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.

Description

Technical field: [0001] The invention relates to a method for obtaining haploid plants of calla lily (Zantedeschia aethiopica) through anther culture, belonging to the technical field of flower breeding. Background technique: [0002] Calla lily (Zantedeschia aethiopica) is a perennial bulbous flower of the genus Zantedeschia in the family Araceae. It is an excellent fresh-cut flower and potted flower; the traditional variety of calla lily has white flowers, but there are other gorgeous flowers in this species currently on the market. Varieties of colorful flowers, calla lilies with high purity and bright white or bright colors and elegant shapes occupy an important position in the flower market. [0003] In the prior art, a pedigree method is used to obtain a genetically stable pure line of calla lily, and the method requires 6-7 generations (15-20 years) to obtain a genetically stable pure line of calla lily. This method not only takes a long time, but also costs a lot. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 张乐民王世敏郑思乡
Owner YUNNAN UNIV
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