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Method for enrichment of n-3 polyunsaturated fatty acid glyceride from fish oil

A technology for unsaturated fatty acids and fatty acid glycerides, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, fermentation, etc., can solve problems such as esterification and enrichment, achieve good food safety, and is conducive to human digestion and absorption. The effect of good medical and health care

Inactive Publication Date: 2009-01-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the separation of EPA and DHA in fish oil and the enrichment of esterification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Accurately weigh 10 g of the refined fish oil sample, add 150 mL of 2mol / L NaOH aqueous ethanol solution, the ethanol / water ratio (v / v) is 6:1, protect with nitrogen, saponify at 50 °C for 60 min, add 100 mL of n-hexane to extract the unsaponified The hydrated layer was acidified with 100 mL of 3mol / L HCl, and then the free n-3PUFA hydrolyzed was extracted with 100 mL of n-hexane × 3, washed with water until neutral, and passed through anhydrous Na 2 SO 4 , N 2 The solvent is blown dry to obtain n-3PUFA, the hydrolysis rate of fish oil is 95.5% by chemical hydrolysis, the yield of EPA is 92%, and the yield of DHA is 90%.

[0023] 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after the hydrolysis of the fish oil was subjected to chromatographic separation on a C18 column, the mobile phase was methanol / water, 70-100% methanol gradient elution was performed, and the eluent was collected by sections, using Mass spectrometry quickly analyzes the chromat...

Embodiment 2

[0026] Accurately weigh 10g of the refined fish oil sample, add 150mL of 2mol / L NaOH ethanol aqueous solution, the ethanol / water ratio (v / v) is 9:1, nitrogen protection, saponify at 60°C for 90min, add 100mL of n-hexane to extract unsaponified The hydrated layer was acidified with 100 mL of 3 mol / L HCl, and then the free n-3PUFA hydrolyzed by n-hexane was extracted with 100 mL × 3 of n-hexane, washed with water until neutral, and passed through anhydrous Na 2 SO 4 , N 2 The n-3PUFA is obtained by drying the solvent, the hydrolysis rate of fish oil is 92% by chemical hydrolysis, the yield of EPA is 90%, and the yield of DHA is 88%.

[0027] 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after the hydrolysis of the fish oil was subjected to chromatographic separation on a C18 column, the mobile phase was methanol / water, 70-100% methanol gradient elution was performed, and the eluent was collected by sections, using The chromatographic samples were quickly an...

Embodiment 3

[0030] Accurately weigh 10 g of the refined fish oil sample, add 150 mL of 2mol / L NaOH aqueous ethanol solution, the ethanol / water ratio (v / v) is 9:1, fill with nitrogen for protection, saponify at 50 ° C for 90 min, add 100 mL of n-hexane to extract the unsaponified The hydrated layer was acidified with 100 mL of 3mol / L HCl, and then the free n-3PUFA hydrolyzed was extracted with 100 mL of n-hexane × 3, washed with water until neutral, and passed through anhydrous Na 2 SO 4 , N 2 The solvent is dried to obtain n-3PUFA, the hydrolysis rate of fish oil is 94% by chemical hydrolysis, the yield of EPA is 91%, and the yield of DHA is 89%.

[0031] 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after the hydrolysis of the fish oil was subjected to chromatographic separation on a C18 column, the mobile phase was methanol / water, 70-100% methanol gradient elution was performed, and the eluent was collected by sections, using The chromatographic samples were quickl...

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Abstract

The invention relates to a method for enriching n-3 polyunsaturated fatty acid (n-3PUFA) from fish oil, which belongs to the food organism and functional health product technical field. The method takes the fish oil as a raw material, uses the chemical hydrolysis method to hydrolyze the fish oil, respectively obtains high-purity eicosapentaenoic acid (EPA) and high-purity docosahexaenoic acid (DHA) from the hydrolyzed fish oil through separation and purification, uses glycerol and the purified EPA or DHA as raw materials and utilizes lipase to respectively catalyze and synthesize an EPA glyceride product or a DHA glyceride product in an organic phase. The products are mainly n-3PUFA triglyceride; the purity of the products is high; and the concentration of EPA triglyceride in the EPA glyceride is between 90 and 95 percent, and the concentration of DHA triglyceride in the DHA glyceride is between 92 and 94 percent. The high-purity EPA triglyceride product and the high-purity DHA triglyceride product prepared by the method well improve the application pertinence of different health care efficacies of natural fish oil products and improve the functional health care value of the fish oil products.

Description

technical field [0001] The invention provides a method for enriching n-3 polyunsaturated fatty acid glycerides from fish oil. Background technique [0002] The n-3 polyunsaturated fatty acids (n-3PUFA) in fish oil have important biological significance in the human body, characterized by the physiological activities of the fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) It has been clear that EPA has physiological activities such as preventing coronary heart disease, lowering blood pressure, eliminating fatigue, preventing atherosclerosis and cerebral thrombosis, and anti-cancer. DHA can significantly promote the intellectual development of infants, improve brain function, and improve memory. EPA and DHA It is a good base for the production of cardiovascular disease prevention and infant educational foods. [0003] EPA and DHA in natural fish oil mainly exist in the form of glycerides, but the content of EPA and DHA is relatively low (20% to 30%), and...

Claims

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Application Information

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IPC IPC(8): C12P7/64C07C57/02C07C51/48
Inventor 陈洁何志勇陈小娥陶冠军唐学燕全文琴
Owner JIANGNAN UNIV
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